'Imaging cellular and molecular biological function' provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.

Zusammenfassung

Imaging Cellular and Molecular Biological Function provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basic through to complex overview of method and protocols, and there is also practical and detailed how-to content on important, but rarely addressed topics. This first edition features all-colour-plate chapters.

The philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.

Inhalt
Considerations for Routine Imaging.- Entering the Portal: Understanding the Digital Image Recorded Through a Microscope.- Quantitative Biological Image Analysis.- The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics.- Design and Function of a Light-Microscopy Facility.- Advanced Methods and Concepts.- Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls.- Quantitative FRET Microscopy of Live Cells.- Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells.- Single Fluorescent Molecule Tracking in Live Cells.- From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins.- Cutting-Edge Applications and Utilities.- Towards Imaging the Dynamics of Protein Signalling.- New Technologies for Imaging and Analysis of Individual Microbial Cells.- Imaging Parasites in Vivo.- Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells.- High-Throughput/High-Content Automated Image Acquisition and Analysis.- Cognition Network Technology A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents.- High-Content Phenotypic Cell-Based Assays.