This volume explores the latest technologies for RNA analysis, covering fundamental techniques and innovative approaches that expand our understanding of RNA biology. The chapters in this book are organized into four parts: reverse transcription and RNA capture methods; high-throughput sequencing methods; RNA maturation: splicing, polyadenylation, and epitranscriptomic modifications; and RNA surveillance: nonsense-mediated mRNA decay. These chapters aim to provide detailed protocols for select techniques used by molecular and computational biologists to isolate, amplify, detect, modify, and analyze RNA to gain insight into a wide range of cellular functions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Analysis: Methods and Protocols is a valuable resource for students, technicians, and senior scientists who want to learn more about this dynamic field of RNA research.