A NATO Advanced study Institute on "Receptors, Membrane Trans­ port and Signal Transduction", was held on the Island of Spet­ sai, Greece, from August 16-27, 1988, in order to consider re­ cent developments in membrane receptor research, membrane trans­ port and signal transduction mechanisms. These topics were put in the larger context of current knowledge on the structure and function of membranes; connections between different fields of research were established by in-depth dis­ cussions of energy transduction and transport mechanisms. The general principles of regulation by signal transduction and protein phosphorylation/dephosphorylation were presented in the context of specific cellular processes. Discussions included al­ so the role of protein tyrosine kinases which are structurally related to oncogene products and, therefore, implicated in va­ rious aspects of cell development and transformation. This book presents the content of the major lectures and a se­ lection of the most relevant posters presented during the course of the Institute. The book is intended to make the proceedings of the Institute accessible to a larger audience and to offer a comprehensive account of those topics on receptors, membrane transport and signal transduction that were discussed extensive­ ly during the course of the Institute. February 1989 The Editors CONTENTS I. G-PROTEINS, ADENYLATE CYCLASE AND PROTEIN PHOSPHORYLATION Selective regulation of G proteins by Cell surface receptors ......... .

Inhalt

I. G-Proteins, Adenylate Cyclase and Protein Phosphorylation.- Selective regulation of G proteins by Cell surface receptors.- Regulation of adenylate cyclase in mammalian cells and Saccharomyces cerevisiae.- Protein kinases, Protein phosphatases and the regulation of glycogen metabolism.- Phosphorylase kinase and protein kinase C:Functional similaritie.- The use of specific antisera to locate functional domains of guanine nucleotide binding proteins.- Calcium inhibits GTP-binding proteins in squid photoreceptors.- Degradation of the invasive adenylate cyclase toxin of bordetella pertussis by the eukaryotic target cell-lysate.- Identification and characterization of adenylate cyclases in various tissues by monoclonal antibodies.- The role of G-proteins in exocytosis.- Hydrophobic interactions in the calcium-and phospholipid dependent activation of protein kinase C.- Activation of transducing by aluminum or beryllium fluoride complexes.- II. Membrane Receptors and Neurotransmitters.- Glutamate receptors and glutamatergic synapses.- Mechanisms of glutamate exocytosis from isolated nerve terminals.- Characteristics of the epidermal growth factor receptor.- Three-dimensional structural models for EGF and insulin receptor interactions and signal transduction.- Potentiation of neurotransmitter release coincides with potentiation of phosphatidyl inositol turnover - A possible in vitro model for long term potentiation (LTP).- Purification and localization of kainate binding protein in pigeon cerebellum.- The Norepinephrine analog meta-iodo-benzylguanidine (MIBG) as a substrate for mono (ADP-ribosylation).- The synaptic vesicle vesamicol (AH5183) receptor contains a low affinity acetylcholine binding site.- Purification of the D-2 dopamine receptor and characterization of its signal transduction mechanism.- Downregulation of M1 and M2 muscarinic receptor subtypes in Y1 mouse adenocarcinoma cells.- Uptake of GABA and L-glutamate into synaptic vesicles.- Deactivation of laminin-specific cell-surface receptors accompanies immobilization of myoblasts during differentiation.- III. Membrane Transport and Bioenergetics.- Signal Transduction in Halobacteria.- Control of bacterial growth by membrane processes.- Carbonylcyanide-3-chlorophenylhydrazone, a prototype agent for the selective killing of cells in acidic regions of solid tumours.- Ca2+ and pH interactions in thrombin stimulated human platelets..- Structure-function relationships of the pCloDF13 encoded BRP.- Binding of a Bacillus Thuiingiensis delta endotoxin to the midgut of the tobacco hornwoim (Manduca sexta).- Functional reconstitution of photosynthetic reaction centre complexes from Rhodopseudomonas Palustris.- Na+/H+ exchange in cardiac cells: Implications for electrical and mechanical events during intracellular pH changes.- Receptor-mediated inhibition of reproductive activity in a schistosome-infected freshwater snail.- NMR study of gramicidin cation trasnport across and integration into a lipid membrane.