This book features targeting protocols from different systems, including animal, plant, fungal, and protist models. It provides insights into the intricate challenges presented by these diverse systems. In this new edition, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Coverage places emphasis on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.

The authors provide state-of-the-art information on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available.