Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.

Inhalt

The antigen-antibody reaction; production of polyclonal antibodies; production of monoclonal antibodies; modification of antibodies; separation techniques for heterogeneous immunoassays; properties of antibodies and general considerations on reagnet optimization; radio-labelled immunoassay; heterogeneous enzyme immunoassay including enzyme amplification; homogeneous enzyme mediated immunoassays including enzyme, substrate, cofactor amd inhibitor labels; fluoro-immunoassay including time resolved flourescence; homogeneous fluoro-immunoassay including fluorescence polarization and fluorescence excitatin transfer; phosporescence immunoassay; luminescence immunoassays including enhanced systems; electrometric immunoassays; alternate optical immunoassays including evanescent wave - surface plasma resonance; multilayer thin film carrier immunoassay systems; dry reagent carrier immunoassay systems; immunochromatographic immunoassays; immunoconcentration immunoassay; radial partition immunoassay; data handling for immunoassay.