This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books.
It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Inhalt

Facilities and Technology Requirements for Laboratory Operations.- Laboratory Facilities, Operation, and Management.- Media Preparation and Handling.- Sterile Techniques.- Basic Plant Regeneration and Plant Propagation Procedures.- Micropropagation by Proliferation of Axillary Buds.- Adventitious Shoot Proliferation.- Plant Regeneration by Organogenesis from Callus and Cell Suspension Cultures.- Plant Regeneration from Callus and Cell Suspension Cultures by Somatic Embryogenesis.- Direct Somatic Embryogenesis.- Conifer Somatic Embryogenesis, Embryo Development, Maturation Drying, and Plant Formation.- Applications of Organ Culture Systems.- Meristem Culture for Virus Elimination.- Embryo Culture and Embryo Rescue for Wide Cross Hybrids.- Anther Culture for Doubled Haploid Production.- Microspore Culture for Haploid Plant Production.- Protoplasts and Transformation Procedures.- Protoplast Isolation and Culture.- Agrobacterium-Mediated Transformation.- Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment.- Microtechniques and Analytical Procedures.- Botanical Microtechnique for Plant Cultures.- Transmission and Scanning Electron Microscopy for Plant Protoplasts, Cultured Cells and Tissues.- Indirect Immunofluorescence Techniques for Cultured Plant Cells and Protoplasts.- Staining Procedures for Chromosome Analysis.- Enzyme-Linked Immunosorbent Assay (ELISA) Methods to Certify Pathogen (Virus)-Free Plants.- Applications of Polymerase Chain Reaction (PCR) to Plant Genome Analysis.