This volume presents a comprehensive collection of protocols and techniques commonly used in research on plant-biotic interactions. Chapters guide readers through general plant-pathogen interactions, RNA interference (RNAi) in Okra, spot inoculation of begomoviruses, natural transmission, RNA viruses, enzyme-linked immunosorbent, reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (RT-LAMP), northern blot hybridization, and next-generation sequencing (NGS) for in-depth analysis of viral populations and mutation patterns. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights **tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols aims to be comprehensive guide for **researchers in the field.