The development of effectively targeted and efficiently delivered nucleic acids promises important advances in the diagnosis and treatment of human malignancies. In Nucleic Acid Therapeutics in Cancer, leading researchers, academic physicians, and clinicians clearly and succinctly review the important issues underlying the therapeutic use of nucleic acid-mediated gene silencing. Topics range from basic methodology and delivery to targeting methods and specific clinical targets. The authors thoroughly explain the latest developments in RNA biology, as well as the underpinnings of RNA interference, oligodeoxynucleotide delivery into cells, and strategies for targeting these molecules to accessible regions within the mRNA. They also provide some examples of how these new therapeutic compounds are being used clinically.
Concise yet highly readable, Nucleic Acid Therapeutics in Cancer offers those who require a cutting-edge critical synthesis not only a state-of-the-art account of this promising field, but also an illuminating introduction to their emerging therapeutic uses.

Klappentext

A cutting-edge review of the important issues underlying the therapeutic use of nucleic acid-mediated gene silencing. Topics range from basic methodology and delivery to targeting and clinical targets. The authors thoroughly explain the latest developments in RNA biology, as well as the underpinnings of RNA interference, oligodeoxynucleotide delivery into cells, and strategies for targeting these molecules to accessible regions within the mRNA. They also provide some examples of how these new therapeutic compounds are being used clinically.

Inhalt

Part I. Introduction and Perspective Antisense Methodology: An Assessment After 25 Years Fritz Eckstein Nucleic Acid Therapeutics: An Introduction Alan M. Gewirtz Part II. Basic Methodology Targeted Genome Modification Via Triple Helix Formation Faye A. Rogers and Peter M. Glazer Therapeutic Applications of Ribozymes John J. Rossi Use of Catalytic DNA in Target Validation and Therapeutics Lun-Quan Sun Targeted Destruction of Small, Stable RNAs: Principles Applicable to Antisense Therapies David A. Dunbar and Susan J. Baserga Mechanism of Action of Antisense RNA in Eukaryotic Cells Zuo Zhang and Gordon G. Carmichael Part III. Delivery The Transport of Oligonucleotides Into Cells R. L. Juliano Peptide-Mediated Delivery of Antisense Oligonucleotides and Related Material Eric Vivès, Jean Philippe Richard, and Bernard Lebleu Molecular Vectors for Gene Delivery to Cancer Cells Guy Zuber, Jean-Serge Remy, Patrick Erbacher, Pascale Belguise, and Jean-Paul Behr Part IV. Targeting Considerations on the Design of Antisense Oligonucleotides Rosel Kretschmer-Kazemi Far, Jens M. Warnecke, and Georg Sczakiel Identification of Hybridization Accessible Sequence in Messenger RNA Lida K. Gifford, Ponzy Lu, and Alan M. Gewirtz Part V. Clinical Targets Nucleic Acids As Gene Targeting Therapeutics Joanna B. Opalinska and Susan E. Shetzline LY900003 (Isis 3521) and G3139 (Genasense; Oblimersen): Phosphorothioate Antisense Oligonucleotides With Pleiotropic Mechanisms of Action C. A. Stein, Nathalie Dias, Luba Benimetskaya, Jan S. Jepsen, Johnathan C. H. Lai, and Anthony J. Raffo Antisense Protein Kinase A-RIa Restores Normal Signal Transduction Signatures to Inhibit Tumor Growth Yoon S.Cho-Chung Index