Proceedings of the NATO Advanced Research Workshop on Mechanism of Action of the Nicotinic Acetylcholine Receptors held on the Island of Santorini, Greece, May 19-23, 1986

Inhalt

Content.- I. Molecular Structure.- Three-Dimensional Structure of the Acetylcholine Receptor.- Ultrastructural Analysis of the Native Acetylcholine Receptor.- Probing Acetylcholine Receptors with Monoclonal Antibodies.- Acetylcholine Receptor Conformation Probed by Subunit-Specific Monoclonal Antibodies.- What can we Learn About the Acetylcholine Receptor from Synthetic Peptides?.- II. Biophysical and Biochemical Studies of AChR Topography.- Topography of the Acetylcholine Receptor as Revealed by Fluorescence Energy Transfer.- Infrared Studies of the Acetylcholine Receptor Structure.- Monoclonal Antibodies as Probes of Acetylcholine Receptor Function.- Antigenic Specificity of Monoclonal Antibodies Against Torpedo 93 and Human Acetylcholine Receptor, and Use of these Anti-bodies to Investigate Myasthenia gravis.- Tertiary Structure of the Nicotinic Acetylcholine Receptor Probed by Photolabeling and Protein Chemistry.- A Structural Model of the Ion Channel of the Nicotinic Acetylcholine Receptor.- III. Ligand Binding, Ion Flux Studies and Reconstitution.- A General Treatment of Ligand Binding to the Acetylcholine Receptor.- Lipid-Protein Interactions and Acetylcholine Receptor Function in Reconstituted Membranes.- Regulation of Intercellular Signal Transmission. New Approaches to Chemical Kinetic Measurements on Cell Surfaces.- Hysteresis and Channel Properties of the Acetylcholine Receptor from Torpedo Californica.- IV. Electrophysiological Studies.- States of the Nicotinic Acetylcholine Receptor: Enumeration, Characteristics and Structure.- Function of Mammalian Nicotinic Acetylcholine Receptors.- Single Channel Studies of Acetylcholine Receptors Covalently Alkylated with Acetylcholine.- Effects of Allosteric Ligands on the Gating of Single Channel Currents in BC3H-1 Cells.- On the Role of Trophic Factors vs. Muscle Activity in the Development of Acetylcholine-Receptor Ion Channels in Rat Endplates.- Molecular Electrophysiology of Cloned AChR Channels Expressed in Xenopus Oocytes.- V. AChR Associated Proteins.- The Transglutaminase of Acetylcholine Receptor Rich Membranes.- Association of the Postsynaptic 43K Protein with Sites of Clustered and Diffusely-Distributed Acetylcholine Receptor.- cAMP-Dependent Phosphorylation of the Nicotinic Acetylcholine Receptor: Characterization of the Protein Kinase in Torpedo Electric Organ, Lack of Correlation in Myotubes Between Increased Intracellular Levels of cAMP and Influx of Monovalent Ions, Block of Influx by Forskolin Independently of cAMP Levels.- Common Antigenic Determinants Between Acetylcholine Receptor and Protozoan Membranes.- VI. Ganglionic and Brain AChR.- Identification and Reconstitution of a Neuronal Acetylcholine Receptor from Insects.- Studies on the Nicotinic Cholinergic Receptor of Sympathetic Neurones.- Radioligand Labelling of Nicotinic Receptors in Mammalian Brain.- VII. Molecular Genetics.- Molecular Biology of Muscle and Neural Acetylcholine Receptors.- Molecular Biology of Nicotinic Acetylcholine Receptors from Chicken Muscle and Brain.- Acetylcholine Receptor Related Genes Expressed in the Nervous System.- Expression Cloning and Fusion Proteins as Tools to Study Receptor Structure.- Establishing a System for the Stable Expression of Torpedo Acetylcholine Receptors.- Characterization of the mRNA and the Gene of a Putative Neuronal Nicotinic Acetylcholine Receptor Protein from Drosophila.- VIII. Structural Models.- Review and Revision of Structural Models for the Transmembrane Portion of the Acetylcholine Receptor Channel.- A Structural and Dynamic Model for the Nicotinic Acetylcholine Receptor.- Authors Index.