Mycorrhiza - symbiotic associations between plant roots and fungi - play a major role in many fundamental plant functions such as mineral nutrition or stress resistance. As the link between plants and the soil, mycorrhiza are now of great interest for developing new strategies in sustainable agriculture. Since they allow a decreased use of fertilizer and pesticides, negative impacts on the environment can be minimized. With contributions from renowned international scientists, this manual offers a great variety of practical protocols for analyzing mycorrhiza, including the latest molecular, biochemical, genetical, and physiological techniques.

Inhalt

MYCORRHIZA MANUAL (Springer Lab Manual), Varma Ed. List of Contributors-Preface-Foreword, S. GIANINAZZI.-1. Mycorrhizae, the Friendly Fungi: What We know, What Should We Know and How Do We Know?, A. VARMA.-2. Approaches to the Study of Interactions Between VA Mycorrhizal Fungi, S. JUNIPER, L. K. ABBOTT and F. JAYASUNDARA.-3. Application of Molecular Techniques to Ecological Studies of Symbioses in Actinorhizal Plant, C.T. WHEELER, N.R. McEWAN, A. SELLSTEDT and G. SANDSTRÖM.-4. Characterizing Effects of Mycorrhizal Fungi on Plant Population Structure, R.T. KOIDE and D.L. SHUMWAY.-5. Evaluation of Vesicular-Arbuscular Mycorrhizal Colonisation, S. DICKSON and S.E. SMITH.-6. Mycorrhizosphere Microorganisms: Screening and Evaluation, K.G. MUKERJI and A. VARMA.-7. Methods for Cultivating Edible Ectomycorrhizal Mushrooms, I.R. HALL and Y. WANG.-8. Isolation and Regeneration of Protoplasts from Ectomycorrhizal Fungi, R. HAMPP, C. STÜLTEN and U. NEHLS.-9. Electrophoretic Analytical Technique for the Genetic Identification of Mycorrhizal Fungi of the Genus Tuber and Their Mycelium and Mycorrhiza, S. URBANELLI, P. SALLICANDRO AND E. DE VITO.-10. The Use of Polytetrafluoroethylene (PTFE) Hydrophobic Membranes to Study Transport of N15 by Mycorrhizal Hyphae, B. FREY, I. BRUNNER, P. CHRISTIE, A. WIEMKEN and P. MÄDER.-11. Application of Methods of Quantitative Histochemistry on Mycorrhizal Roots, R. HAMPP, C. SCHAEFFER and M. GUTTENBERGER.-12. Measuring Production and Activity of Plant Cell Wall-Degrading Enzymes in . Ectomycorrhizal Fungi, M. BURKE and J.W.G. CAIRNEY.-13. Biochemical Analysis of Polygalacturonases by Ericoid Mycorrhizal Fungi, S. PEROTTO, V. COMETTI, J.D. COISSON, I. PERUGINI and P. BONFANTE.-14. Methods for Determining Phosphatase Activities in Ectomycorrhizal Fungi, M. TIBBETT, S. M. CHAMBERS and J. W. G. CAIRNEY.-15. Determination of Glutathione, Glutathione Disulphide and Two Related Enzymes-Glutathione Reductase and Glucose-6-Phosphate Dehydrogenase inFungal and Plant Cells, I. KRANNER.-16. Identification and Characterzation of Siderophores of Mycorrhizal Fungi, K. HASELWANDTER and G. WINKELMANN.-17. Polyclonal Antibodies for the Detection of AM Fungi, A. HAHN, C. GÖBEL and B. HOCK.-18. Monoclonal Antibodies for the Identification of AM Fungi, C. GÖBEL, A. HAHN, T. GIERSCH and B. HOCK.-19. Targeted Approaches for Detecting Changes in Protein Expression with Mycorrhiza Development: Hydrolytic Isoenzymes Analyses and Immunological Detection of Known Protein in Root Extracts, E. DUMAS-GAUDOT, B. DASSI, S. SLEZACK, V. GIANINAZZI-PEARSON and S. GIANINAZZI.-20. Untargeted Approaches to the Analysis of Gene Expression in Arbuscular Mycorrhiza: Analyses of Polypeptides (in Vivo and in Vitro) and of Translated mRNA Products, E. DUMAS-GAUDOT, A. SAMRA, V, GIANINAZZI-PEARSON and S. GIANINAZZI.-21. The Molecular Route to Understanding VAM Symbiosis. G. DELP, G. M. ROSEWARNE and S. J. BARKER.-22. Molecular Approaches to Investigate Biodiversity in Mycorrhizal Fungi, L. LANFRANCO, S. PEROTTO, S. LONGATO, A. MELLO, V. COMETTI and P. BONFANTE.-23. DNA Isolation and Development of Molecular Tools for Identification and Detection of VA Mycorrhizal Fungi, A. ZEZE, K. OPHEL-KELLER and S.E. SMITH.-24. PCR in Studies of AM Fungi: from Primers to Application, D. van Tuinen, B. Zhao and V. Gianinazzi-Pearson.-25. PCR Cloning of Genes from Arbuscular Mycorrhizal Fungi, P. FRANKEN, B. RESSIN, L. LAPOPIN, V. GIANINAZZI-PEARSON and S. GIANINAZZI.-26. RAPDs and ITS Analysis of Orchid Mycorrhizal Fungi, G.C. SAUNDERS and S. J. OWENS.-27. Isolation and Partial Characterization of Double-Stranded RNA-Containing Viruses of Orchid Mycorrhizal Fungi, J.D. JAMES, G.C. SAUNDERS and S.J. OWENS.-28. Identification and Characterization of Genes by Mutant Complementation, U. NEHLS and R. HAMPP.-29. Spatial Regulation of Genes in Ectomycorrhiza: mRNA in Situ Localization and beta-Glucuronidase Detection, D. TAGU and S. HAWKINS.-30. Genomic