This second edition volume expands on the previous edition with updated protocols on basic techniques of radioligand binding, allosteric modulation, visualization and antibody use, and autoradiography and non-neuronal acetylcholine detection. The updated chapters also cover the latest fluorescent techniques including fluorescence anisotropy, and provide updates on current system measurements of heart contractility and gastrointestinal tract motility to provide readers with a comprehensive picture of current muscarinic receptors from molecular to systemic levels. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Muscarinic Receptors - From Structure to Animal Models, Second Edition is a valuable resource for scientists who are interested in using the latest methodologies to study muscarinic receptors quickly, easily, and correctly.