This edited work presents useful methods in experimenting in the area of Bioprocessing and Biotechnology. The four sections cover the area of Bioprocess, Whole Cells & Isolated Biocatalyst, Characterization of Biochemical Products and Cell Isolation & Culture. Its enable researchers to choose a suitable method and plan their experiments in details.

The main focus of this book is to provide step by step method to young researchers, especially in the new research areas. Among the latest areas are the isolation of novel strain or enzyme by metagenomic approaches and taming procedure in the laboratories, development of novel, the cheap and non-toxic catalyst for biodiesel production, and production of micro-fibrillated cellulose. An updated method for well-known areas such as immobilization technology, biosensor, and polymerization was also presented. The book also covers in-silico methods such as MATLAB platform to ease researchers. Not to forget, the method in animal and plant culture is also discussed in detail.

The book is written by chapter authors with much expertise in their fields. They have published multiple articles in the index listed journals.

The topic of this book is particularly relevant to young researchers who are struggling to fine-tune their research and do not want to waste their time in optimizing the experiment set up.

Autorentext
Dr. AzuraBinti Amid, a former graduate of the University of Malaya, Kuala Lumpur started her career as a Teacher at Segamat, Johor in 1996. She was appointed as an Assistant Lecturer at the International Islamic University Malaysia in 2001 and later as lecturer atKulliyyah of Engineering (IIUM) in 2011. She obtained the Bachelor of Science with Education (Biology),Master of Science (Molecular Biology in Plant) and Degree of Doctor of Philosophy in Molecular Biology from The University of London, United Kigdom. Currently she is attached as Assoc. Prof. to the Department of Biotechnology Engineering, International Islamic University Malaysia, Kuala Lumpur and Dean of Kulliyyah of Engineering, IIUM.

Inhalt

Preface

1. Site-Directed Mutagenesis on Plasmid Using Polymerase Chain Reaction
   Abdul Aziz Ahmad, Hamzah Mohd Salleh & Ibrahim Ali Noorbatcha

2. Isolation of Halophilic Bacteria
   Nadiah Syuhada Abd. Samad & Azura Amid

3. Construction of Metagenomic DNA Libraries and Enrichment Strategies
   Farah Fadwa Ben Belgacem, Hamzah Mohd Salleh & Ibrahim Ali Noorbatcha

4. Thermotolerant Bacteria Producing Fibrinolytic Enzyme
   Azura Amid & Nurul Aqilah Ab. Shukor

5. Chemical Mutation Method for High CO2-Requiring Mutants of the Cyanobacterium Synechococcus sp. PCC 7002
   Ummi Syuhada Halmi Shari, Azlin Suhaida Azmi & Azura Amid

6. Identification of Fatty Acid Methyl Ester in Palm Oil Using Gas Chromatography-Mass Spectrometer
   Sarina Sulaiman

7. Procedure to Develop Binodal Curve and Phase Diagram for Aqueous Two-Phase System
   Zatul Iffah Mohd Arshad & Azura Amid

8. Technique to Produce Catalyst From Egg Shell and Coconut Waste for Biodiesel Production
   Sarina Sulaiman & Nur Syakirah Talha

9. Carrier-free Enzyme Immobilization by Cross-Linked Enzyme Aggregates (CLEA) Technology
   Faridah Yusof & Soofia Khanahmadi

10. Isolation of Microfibrillated Cellulose (MFC) Via Fungal Cellulases Hydrolysis Combined with Ultrasonication
    Dzun Noraini Jimat & Aviceena

11. Characterization of Electrochemical Transducers for Biosensor Applications
    Farrah Aida Arris, Abdel Mohsen Benoudjit, Fahmi Sanober & Wan Wardatul Amani Wan Salim

12. Polymerization Methods and Characterizations for Poly(Lactic Acid) Based Polymers
    Fathilah Ali & Norshafiq Ismail

13. Polymers in Biosensors
    Jia Jia Long, Abdel Mohsen Benoudjit, Farrah Aida Arris, Fathilah Ali, & Wan Wardatul Amani Wan Salim Characterization of Conformational and Oligomeric States of Proteins Fazia Adyani Ahmad Fuad

14. Skim Latex Serum as an Alternative Nutrition for Microbial Growth
    Vivi Mardina & Faridah Yusof

15. Method for Isolation of Bacteria Strain from Contaminated Soil for Biodegradation of Polycyclic Aromatic Hydrocarbons (PHAs)
    Nooraidah Mustaffa, Olorunnisola Kola Saheed, Parveen Jamal & Abdul Haseeb Ansari

16. Monitoring the Growth of Plant Cells in Suspension Culture
    Noor Illi Mohamad Puad & Tajul Afif Abdullah

17. Culturing and Maintaining Mammalian Cell Culture
    Raha Ahmad Raus, Ghassan Ali Salih & Mustaffa Nammer Shaban

18. Integrated Data Analysis Model for Screening Cell Line Producer
    Nor Fadhillah Mohamed Azmin

Index.