This updated second edition of Molecular Typing in Bacterial Infections, presented in two volumes, covers both common and neglected bacterial pathogenic agents, highlighting the most effective methods for their identification and classification in the light of their specific epidemiology. New chapters have been included to add new species, as well as another view of how bacterial typing can be used. These books are valuable resources for the molecular typing of infectious disease agents encountered in both research and hospital clinical laboratory settings, as well as in culture collections and in the industry. Each of the 21 chapters provides an overview of specific molecular approaches to efficiently detect and type different bacterial pathogens. The chapters are grouped in five parts, covering respiratory and urogenital pathogens (Volume I), and gastrointestinal and healthcare-associated pathogens, as well as a new group of vector-borne and Biosafety level 3 pathogens including adescription of typing methods used in the traditional microbiology laboratory in comparison to molecular methods of epidemiology (Volume II). Comprehensive and updated, Molecular Typing in Bacterial Infections provides state-of-the-art methods for accurate diagnosis and for the correct classification of different types which will prove to be critical in unravelling the transmission routes of human pathogens.

Autorentext

Dr. Ivano de Filippis is a Senior Researcher at Institute for Quality Control of the Oswaldo Cruz Foundation (FIOCRUZ) in Rio de Janeiro, Brazil.  His research field focuses on molecular diagnostic and epidemiology of bacterial and viral diseases and antibiotic resistance mechanisms of bacterial pathogens. Dr. de Filippis received his Ph.D. in Cellular and Molecular Biology from FIOCRUZ in 2005 and a postdoctoral degree at the National Institutes of Health (NIH), USA, with a two-years project on molecular epidemiology of meningococci from 2006 to 2008, with Dr. Rebecca D. Prevots and Dr. Margaret C. Bash. He is a permanent professor at the Post-Graduate Program of Health Surveillance at FIOCRUZ, tutoring national and international post-graduation students on several projects on bacterial meningitis, Sexually Transmitted Diseases and Covid-19 among others. Dr. de Filippis is currently the Research Coordinator at the Institute of Quality control at FIOCRUZ. He participate as a Brazilian consultant for the WHO initiative Defeating Meningitis by 2030 and he holds a patent on rapid methods for bacterial meningitis diagnostic.

Zusammenfassung

Every year new laboratory methods are developed for the identification and classification of infectious disease agents. The number of new approaches to aid epidemiological studies of microbial pathogens is continuously growing. New biotechnology improvements have played a key role in leading to new findings in the fields of laboratory diagnostic and classification of important etiological agents. For each bacterial species, a number of different methods can be used to identify the organism and determine its possible relationship to an epidemic- or outbreak-related clone. Because of the array of different laboratory methods, it is often difficult to choose an effective low-cost method that could be used routinely in laboratories. The need to establish more sensitive methods based on molecular analysis of the microorganism genome has led many laboratories to make large investments in order to achieve results which could have been reached with minor expenses.

Molecular Typing in Bacterial Infections covers common bacterial pathogenic agents, with the most effective methods for their identification and classification in the light of their specific epidemiology. The book will be a valuable resource for molecular typing of infectious diseases agents encountered in both the research and hospital clinical lab settings, as well as culture collections. Each chapter provides an overview of molecular approaches to typing bacterial pathogens. Part I gives a general overview of typing methods used in the traditional microbiology laboratory in comparison to molecular methods of epidemiology. In Part II, the relative strengths and weaknesses of the different methods applicable to the specific agents of infectious diseases are emphasized. Specific emphasis is placed on recent changes and updates in molecular typing.

Inhalt
1. CorynebacteriumVerônica Viana Vieira, Juliana Nunes Ramos, Louisy Sanches dos Santos, Ana Luíza Mattos-Guaraldi
2. Mycoplasma and UreaplasmaKen B. Waites, Li Xiao, Rose M. Viscardi, Sixto M. Leal Jr. and John I. Glass 
3. Neisseria meningitidisC. R. Robert George, Helen Smith and Monica M. Lahra
4. Non-Tuberculous MycobacteriaEmilyn Costa Conceição, Maria Cristina S. Lourenço, Rafael Silva Duarte and Philip Noel Suffys
5. Oral StreptococciMarlise I. Klein
6. Streptococcus pneumoniaeFelipe P. G. Neves and Tatiana C. A. Pinto
7. Neisseria gonorrhoeaeRaphael Cavalcante de Medeiros, Sergio Eduardo Longo Fracalanzza and Raquel Regina Bonelli
8. Streptococcus agalactiae (Group B Streptococcus)Sarah Shabayek
9. TreponemaMayur Shukla, Lara Pereira and Allan Pillay