This volume provides an overview of advanced protocols for vascular biology research, comprising cardiovascular and lymphatic biology. Chapters detail techniques for isolating and characterizing endothelial and mural cell populations, disseminating signaling pathways and protein-protein interactions, constructing organ-on-chip systems, and using animal models and techniques with bench-to-bedside potential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Methods in Cardiovascular and Lymphatic Biology aims to provide protocols and applications that can be used by both new investigators and experienced professionals to advance cardiovascular and lymphatic research.