Macromolecular Crystallography

This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics.
A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.

Inhalt

Preface. List of contributors.- Spatial And Temporal Organisation Of Multiprotein Systems Of Cell Regulation And Signalling: What Can We Learn From Nhej System Of Double-Strand Break Repair?; Qian Wu.- Co-Translational Protein Processing, Folding, Targeting, And Membrane Insertion Of Newly Synthesized Proteins; D. Boehringer, N. Ban.- The Role Of Multiple Sequence Repeat Motifs In The Assembly Of Multi-Protein Complexes; D. Barford.- Cryoelectron Tomography Or Doing Structural Biology In Situ ; W. Baumeister.- Ruvbl1 And Ruvbl2 And Their Complex Proteins Implicated In Many Cellular Pathways; S. Gorynia et al.- The Structural Biology Of Muscle: Spatial And Temporal Aspects; K. Holmes.- Molecular Basis Of Allosteric Transitions: GroEL; A, Horovitz.- Cell Signalling Through Covalent Modification And Allostery; L. N. Johnson.- Combining Cryo-Em And X-Ray Crystallography To Study Membrane Protein Structure And Function; W. Kühlbrandt.- Molecular Mechanisms Of Dna Polymerase Clamp Loaders; B. Kelch et al.- Electron Microscopy Of Macromolecular Machines; Helen R. Saibil.- Assembly And Function Of The Signal Recognition Particle From Archaea; E. Sauer-Eriksson et al.- Structural Studies Of The Functional Complexes Of The 50s And 70s Ribosome, A Major Antibiotic Target; Th. A. Steitz et al.- Proteopedia: Exciting Advances In The 3d Encyclopedia Of Biomolecular Structure; J. Prilusky.- Structure Analysis Of Biological Macromolecules By Small-Angle X-Ray Scattering; D. I. Svergun.- Structural Dynamics Of The Vault Ribonucleoprotein Particle; A. Casañas.- Structural Dynamics Of Picornaviral Rdrp Complexes. Implications For The Design Of Antivirals; N. Verdaguer.- Ribosomes: Ribozymes That Survived Evolution Pressures But Is Paralyzed By Tiny Antibiotics; A.Yonath.-