In Lactoferrin: Interactions and Biological Functions, experimental and clinical investigators join forces to explain our current understanding of lactoferrin, a highly significant protein in medicine and industry. Drawing on many disciplines, the front-line authorities writing here define the structural features of lactoferrin and describe in detail several of its physiological functions. These include the role of lactoferrin in killing bacteria, and its involvement in cell growth and proliferation, in the modulation of immune function, and in iron absorption. Particular attention is given to lactoferrin functions in the regulation of normal and diseased states. New knowledge of the molecular biology of lactoferrin is also included, along with discussions of commercially viable large-scale production techniques and important new industrial applications.

Lactoferrin: Interactions and Biological Functions offers a comprehensive, multidisciplinary view of our present knowledge of lactoferrin and opens the way to the greatly expanded use of lactoferrin as a bioactive reagent in foods and pharmaceuticals. Now the new standard reference in the field, Lactoferrin will prove invaluable to all those working with this important biochemical in both basic and clinical settings.

Klappentext

The number of investigators focusing their attention on lactoferrin has increased dramatically in recent years. Lactoferrin is a protein with more than one known structure and a number of proposed biological functions, including several with important regulatory consequences. In many ways it has been an easy pro­ tein to investigate; however, there have been difficulties under­ standing specific structure / function relationships, particularly as it functions in vivo. Research funding dedicated to this protein has previously been limited, but is now increasing. As lactoferrin begins to emerge formally as a protein of significance to the medi­ and industry, it is more important than ever to coor­ cal profession dinate and integrate research efforts whenever possible and to share the results of these efforts within the expanding array of medical and scientific diSciplines involved. It was our intention to provide a forum to summarize and disseminate the most recent advances in this field. Included in Lactoferrin: Interactions and Biological Functions are selected presentations representing the many disciplines involved in defining lactoferrin function in terms of its known structural features, including its carbohydrate side-chains, receptor binding sites, its capacity to bind different metal ions, and other newly discovered bioactive domains. Several of the possible physiologi­ cal functions of lactoferrin are described and summarized in detail, including the role of laetoferrin in bacterial killing, its in­ volvement in cell growth and proliferation, in the modulation of immune function, and in iron absorption.

Inhalt

Part I: Lactoferrin Structure and Function. Lactoferrin Structure-Function Relationships: An Overview, Jeremy H. Brock. Altered Domain Closure and Iron Binding in Lactoferrin Mutants, H. Rick Faber, Bryan F. Anderson, Heather M. Baker, Tony Bland, Catherine L. Day, Hale Nicholson, Steven Shewry, John W. Tweedie, and Edward N. Baker. Affinity Mass Spectrometry: Probes with Surfaces Enhanced for Affinity Capture (SEAC) of Lactoferrin, Tai-Tung Yip and T. William Hutchens. Part II: Lactoferrin Gene Expression. Variants and Biotechnological Use of the Bovine Lactoferrin-Encoding Gene, Hans-Martin Seyfert, Uta Klußmann, Uta Maria Steinhoff, Jens Vanselow, Dirk Koczan, and Gerd Hobom. Posttranscriptional Regulation of Bovine and Human Lactoferrin: Species Differences and Influence of mRNA Regions, Floyd L. Schanbacher, Surapon Pattanajitvilai, and Margaret C. Neville. Mutagenesis of Human Lactoferrin and Expression in Baby Hamster Kidney Cells, John W. Tweedie, Edward N. Baker, Catherine L. Day, Bhavwanti Sheth, and H. Hale Nicholson. Structural Determination of Two N-Linked Glycans Isolated from Recombinant Human Lactoferrin Expressed in BHK Cells, Dominique Legrand, Valérie Salmon, Bernadette Coddeville, Monique Benaïssa,Yves Plancke, and Geneviève Spik. Distribution of the Iron-Binding Protein Lactoferrin in the Pathological Lesions of Neurodegenerative Diseases, Béatrice Leveugle, Geneviève Spik, Daniel P. Perl, Constantin Bouras, Howard M. Fillit, and Patrick R. Hof. Lactoferrin Almost Absent from Lactating Rat Mammary Gland Is Replaced by Transferrin, Annick Pierce, Hector Escriva, Bernadette Coddeville, Monique Benaïssa, Didier Leger, Geneviève Spik, and Mercedes Pamblanco. Estrogen Regulation of Human Lactoferrin Gene Activity: Transcriptional Synergism Between Estrogen Receptorand Related Orphan Receptor, Christina T. Teng and Nengyu Yang. Expression and Functional Analysis of Recombinant Human Lactoferrin, Pauline P. Ward, Christopher S. Piddington,Grainne Cunningham, Xiaodong Zhou, Roger D. Wyatt, and Orla M. Conneely. Structural and Functional Flexibility of Lactoferrin, Edward N. Baker, Bryan F. Anderson, Heather M. Baker, Rick Faber, Clyde A. Smith, and Andrew J. Sutherland-Smith. Difference in Binding and Fate of Lactotransferrin in Jurkat Human Lymphoblastic T-Cells and in T-47D Human Breast Cancer Cells, Bao Yuan Bi, Elisabeth Elass, Dominique Legrand, Florence Deplace, Geneviève Spik, and Joël Mazurier. The Impact of Phagocyte-Lactoferrin Interactions on Inflammation, Bradley E. Britigan, Troy S. Lewis, Oyebode Olakanmi, Michael L. McCormick, and Rachel A. Miller. Regulation of Lymphocyte Proliferation by Lactoferrin, Jeremy H. Brock and Abdelhakim Djeha. Recombinant Human Lactoferrin and Its Variants: Receptor Binding in Human Intestinal Brush-Border Membranes, Suhasini Iyer, Bo Lönnerdal, Catharine Day, Edward N. Baker, John Tweedie, Tai-Tung Yip, and T. William Hutchens. Part III: Functions Related to Lactoferrin Interactions with Prokaryotic and Eukaryotic Cells. Influence of Lactoferrin on Host-Microbe Interactions, Satyanarayan Naidu and Roland R. Arnold. Bacterial Lactoferrin Receptors in the Neisseriaceae, Robert A. Bonnah, Rong-hua Yu, and Anthony B. Schryvers. Bacteriostatic Effects of Orally Administered Bovine Lactoferrin on Intestinal Bacteria in the Gut of Mice Fed Bovine Milk, Susumu Teraguchi, Kouichirou Shin, Kazuhiro Ozawa, Satoko Nakamura, Yasuo Fukuwatari, Seiichi Shimamura, and Mamoru Tomita. Functional Significance of the Binding of Lactoferrin to DNA, Philip Furmanski, Jianglin He, Li Ying, Joann Bezault, Ramesh Bhimani, and Kinfan Ho. Specific Binding of Ferrilactoferrin and Ferritransferrin in the Protozoan Leishmania chagasi, Michael L. McCormick, Mary E. Wilson, Troy S. Lewis, Robert W. Vorhies, and Bradley E. Britigan. Part IV: Lactoferrin Metabolism. Observations on the Metabolism and Cellular Interactions of Lactoferrin, Erwin Regoeczi. Lactoferrin as a Possible