This book embarks on an enlightening journey through the molecular landscape of collagen mimic peptides, shedding light on their significance and potential. In the intricate realm of biomolecules, collagen's preeminence as the most abundant protein in the human body, forming the foundational scaffolding of tissues and organs, renders it a subject of profound scientific interest. It explores diverse facets related to collagen mimic peptides, spanning aspects such as bioinformatic analysis, synthetic strategies, pathological collagen targeting, and the construction of homotrimeric, heterotrimeric and self-assembled peptide models. Furthermore, it provides an exhaustive investigation into biophysical techniques, encompassing Circular Dichroism (CD), X-ray crystallography, Nuclear Magnetic Resonance (NMR), Fluorescence, and Raman spectroscopy, thereby empowering researchers to unravel the structural intricacies of these peptides. The book unravels the profound implications of collagen mimic peptides across a spectrum of scientific domains, including protein science, biomaterials, bioanalysis, and beyond. Its accessibility and insights cater not only to seasoned researchers but also to undergraduate and graduate students eager to delve into the complexities of this fundamental protein.

Autorentext

Prof. Jianxi Xiao received his bachelor's degree from Wuhan University in 2003 and Ph.D. degree from Rutgers University-New Brunswick, New Jersey in 2009. He continued his postdoc studies at Rutgers University and got a full professor position in College of Chemistry and Chemical Engineering at Lanzhou University, Gansu, China in 2012. Dr. Xiao's research interests focus on biophysical characterization of collagen mimic peptides by utilizing an integrated approach of NMR, Fluorescence, Computational simulation, Bioinformatics and Biochemistry. His work has greatly enhanced our understanding of the structure, function and pathology of collagen, and has been published in esteemed journals such as the Journal of American Chemical Society and Journal of Biological Chemistry. Dr. Xiao is reviewer of several international journals and invited speaker of several international conferences.

Klappentext

This book covers all the key biophysical techniques needed to characterize collagen-mimetic peptides such as CD, X-ray, NMR and fluorescence. It also elaborates how the thermal stability, structure, and dynamics, as well as quantitative information on collagen model peptides are obtained. Pursuing a holistic approach, the book establishes a fundamental framework, while emphasizing the potential applications of each technique in detail. This approach helps readers from different disciplines recognize the essential aspects of and milestones in the biophysical techniques developed specifically for collagen peptides.

The book is intended for undergraduate and graduate students who are interested in collagen, researchers investigating collagen function and diseases, and material scientists working on biomimetic materials.

Inhalt

Introduction to collagen.- Structure of collagen triple helix.- Bioinformatics-aided Design of collagen mimic peptides.- Synthesis of collagen peptides.- CD characterization of triple helix stability.- Structural insights from X-ray crystallography.- Conformational and dynamic characterization by NMR spectroscopy.- Fluorescence detection of collagen biomarkers.- Synthesis and characterization of heterotrimeric models of collagen.- Self-assembly of collagen mimic peptides.- Concluding remarks.