Inhalt

I Flow Cytometry.- 1 Operation of a Flow Cytometer.- II Immunofluorescence.- 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies.- 3 Immunofluorescence: Basic Considerations.- 4 Multicolor Immunofluorescence Analysis.- 5 Combined Intracellular and Surface Staining.- 6 Scatchard Analysis by Flow Cytometry.- III DNA and Proliferation.- 7 Preparation and Staining of Cells for High-Resolution DNA Analysis.- 8 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression.- 9 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique.- IV Cellular Activation and Biochemistry.- 10 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium.- 11 Biochemical Parameters of Cell function.- 12 Molecular Analysis of Transcriptional Control: The FACS- Galactosidase Assay.- 13 Ligand Acidification by Nonadherent Cells.- V Cell Sorting.- 14 Powerful Preselection.- 15 High Gradient Magnetic Cell Sorting.- 16 Setup of a Flow Sorter.- 17 Sorting of Rare Cells.- 18 Fluorescence-Activated Chromosome Sorting.- 19 Flow Cytometry and Sorting of Plant Chromosomes.- 20 Large Particle Sorting.- VI Safety.- 21 Biological and Laser Safety.- List of Suppliers.