Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology. This book presents an up-to-date overview of current ideas and methods in these domains. It consists of three parts. Part I, "Membrane Dynamics and Function", deals with adhesion molecules, membrane pump dynamics, membrane fusion and endocytosis, membrane potential, T-cell homing and cytoskeleton, etc. The second part focuses on problems of cell proliferation, chromosome analysis and sorting, apoptosis, imaging cytometry of fixed and living cells, image analysis for gene mapping from flow-sorted chromosomes, chromatin organization, and liposome-mediated delivery of DNA binding drugs. The third part covers data management systems, cell sorting techniques and microscopy.

Inhalt

I: Membrane Dynamics and Function.- Analysis of CD28 Interactions with Its Cognate Counter-Receptors CD80 and CD86.- Membrane Transport Dynamics.- Endogenous Lectins in Circulating Cells and Their Glycosylated Ligands: Their Role in T Cell Homing.- Use of Different Cytometric Techniques to Study the Cytotoxic Interaction between Human Natural Killer Cells and K562 Target Cells.- In Vitro Reconstitution of Early Endosome Membrane Dynamics.- Change in Membrane Fluidity After Pinocytosis of a New cytokine IK on Resting and Activated T cells.- II: Cell Proliferation and Gene Expression.- Cell Cycle and Cell Proliferation Markers.- Advances in Flow Cytogenetics: Progress in the Development of a High Speed Optical Chromosome Sorter Based on Photoinduced Adduct Formation Between psoralens and chromsomal DNA.- Analysis of Cell Death by Flow Cytometry.- Molecular Cytogenetics: Uses of Flow Sorted Chromosomes, Fluorescence in situ Hybridisation (FISH) and Digital Microscopy for the Analysis of Genomes.- DNA Topoisomerases as Drug Targets and Cell Cycle Checkpoint Effector Molecules.- Hemopoïetic Cell Differentiation and Death by Retinoids.- III: Data Management Systems Cell sorting Techniques Microscopy.- Myc in The Control of Proliferation and Apoptosis.- Flow Cytometry: Analyses for All Sizes.- Flow Cytometric Immunophenotyping Using Cluster Analysis and Cluster Editing.- to High-Speed Flow Sorting.- Applications of Near-Field Microscopes to Cell Biology.