Creating bacteria with modified genetic properties allows the specific investigation of these microorganisms. Electrotransformation is a highly efficient and easy to apply technique to introduced genetic material into bacterial cells, with the use of a strong electric field. In the present manual, protocols for the transformation of about 40 strains of bacteria are described. Emphasis is given on the individual critical procedural steps, since the practical details depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows the user to modify and adapt each protocol to his/her own experiments. Bacterial strains with relevance in the food industry and biotechnology, in medical and veterinary fields, agroindustry and environmental sciences are covered.

Klappentext

In this manual, protocols for the transformation of about 40 strains of bacteria are described, with the emphasis placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows users to modify and adapt each protocol to their own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.

Inhalt

I Introduction.- 1 General Principles of Bacteria Electrotransformation: Key Steps.- 2 A Critical Introduction to the Technology of Bacterial Electrotransformation.- 3 Electrotransformation of Escherichia coli.- 4 Transformation of Bacillus subtilis PB1424 by Electroporation.- II Biotechnology and Food Technology.- 5 Clostridium in Biotechnology and Food Technology.- 6 Electrotransformation of Lactococcus lactis.- 7 Electrotransformation of Salmonella typhimurium.- 8 Electroporation of bifidobacteria.- 9 Electrotransformation of Listeria species.- 10 Transformation of Methylobacterium extorquens with a Broad-Host-Range Plasmid by Electroporation.- 11 Electrotransformation of Acidophilic, Heterotrophic, Gramnegative Bacteria.- 12 Acetobacter xylinum - Biotechnology and Food Technology.- 13 Electrotransformation of Sphingomonas pancimobilis.- 14 Bacillus amyloliquefaciens - Production Host for Industrial Enzymes.- III Medical and Veterinary Applications.- 15 Electrotransformation of Yersinia ruckeri.- 16 Electrotransformation of Enterococci.- 17 Prevotella bryantii, P. ruminicola and Bacteroides Strains.- 18 Electrotransformation of Bordetella.- 19 Transformation of Campylobacter jejuni.- 20 Slow-Growing Mycobacteria.- 21 Electrotransformation of Photobacterium damselae subsp. piscicida.- 22 Actinobacillus actinomycetemcomitans: Electrotransformation of a Periodontopathogen.- 23 Francisella in Medical and Veterinary Applications.- 24 Electroporation of the Anaerobic Rumen Bacteria Ruminococcus albus.- 25 Electroporation of Legionella Species.- 26 Electrotransformation of Streptococcus pneumoniae.- IV Plants.- 27 Clavibacter michiganensis - Transformation of a Phytopathogenic Gram-Positive Bacterium.- 28 Electrotransformation of Agrobacterium turnefaciens and A. rhizogenes.- V Environmental Bacteria.- 29 Transformation of the Filamentous Cyanobacterium Fremyella diplosiphon.- 30 Electroporation of Bacillus thuringiensis and Bacillus cereus.- 31 Introduction of Plasmids into Azospirillum brasilense by Electroporation.- 32 Cyanobacteria: Electroporation and Electroextraction.- 33 Electrotransformation of Plasmids into Freshwater and Marine Caulobacters.- Appendices.- Appendix A Plasmid Preparation.- Appendix B Suppliers.