Production and Characterization of Bacterial Thermostable Cellulase

Bhavtosh Kikani

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Project Report from the year 2018 in the subject Biology - Micro- and Molecular Biology, grade: 0, , language: English, abstract:...

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Project Report from the year 2018 in the subject Biology - Micro- and Molecular Biology, grade: 0, , language: English, abstract: Cellulose is one the most abundant carbon sources present on the earth. In the book, the author reported on production optimization and characterization of cellulolytic enzymes by thermophilic bacteria. Various adaptation strategies adapted by thermophilic bacteria are also discussed along with. The thermophilic bacterial strain produced cellulase in the CMC broth, pH 7 containing 0.5% peptone, 0.5% malt extract, 0.2% ammonium sulphate, 0.2% ammonium nitrate and 0.2% NaCl at 50°C. Interestingly, the bacteria could not grow at 37°C, confirming its thermophilic nature. Further, the cellulase was characterized after getting partially purified by ammonium sulphate precipitation method. The partially purified cellulase may be employed to hydrolyze agricultural wastes to produce bioethanol, i.e. biofuels. Thus, the present research may help solve issues of crisis of renewable energy as well as environmental pollution.