This book provides comprehensive knowledge and a detailed step by step description of experimental protocols for the determination of food intake, body weight changes, and some metabolic markers. Chapters are split into two parts detailing experimental diets, monitor food intake, weight gain, evaluate biological samples, predict physiological changes, evaluate bioavailability of bioactive, anamnesis, measure the metabolic rate, assessing the body composition, assessing glucose homeostasis, and monitoring the metabolomic pathways.

Authoritative and cutting-edge, Basic Protocols in Foods and Nutrition aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

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Part I: Animal Experimentation

1. Experimental Diets (Normocaloric and Hypercaloric Diets)

   Laís Marinho Aguiar, Juliana Kelly da Silva Maia, Cinthia Baú Betim Cazarin

2. Measures of Food Intake, Body Weight Gain, and Energy Efficiency in Mice - Cíntia Reis Ballard and Cinthia Baú Betim Cazarin

3. The assessment of glucose homeostasis in rodents: Glucose, Insulin and Pyruvate Tolerance Tests Nathalia R. V. Dragano and Edward Milbank

4. Histopathological Evaluation of Steatohepatitis in Animal Experiments

   Yoshihisa Takahashi, Erdenetsogt Dungubat, Toshio Fukusato

5. Quantification of short-chain fatty acids in feces

   Mirella Romanelli Vicente Bertolo, and Stanislau Bogusz Junior

6. Quantification of non-esterified fatty acids in serum and plasma

   Mirella Romanelli Vicente Bertolo, and Stanislau Bogusz Junior

7. Lipid Peroxidation (TBARS) in biological samples Lilian R. B. Mariutti

8. Extraction of Bile Acids from Biological Samples and Quantification Using Ultra-High-Performance Liquid Chromatography-Orbitrap Mass Spectrometry

   Shota Hori, Hongxia Liu, Riho Yamada, Shun Ichitsubo, Ayana Sakaguchi, Fumika Yokoyama, and Satoshi Ishizuka

9. Glycogen measurement

   Cavaglieri, C.R.; Silva, C.A.; and Bernardes, C.F.

10. Evaluation of the minerals and trace elements in the biological samples

    Duygu Aydemir and Nuriye Nuray Ulusu

11. Microbiome Evaluation

    Alba Rodríguez-Nogales, Antonio Jesús Ruiz-Malagón, Jose Alberto Molina-Tijeras, Maria Elena Rodríguez-Cabezas, Julio Gálvez

12. The GSH colorimetric method as measurement of antioxidant status in serum and rodent tissues

    Milena Morandi Vuolo, Juliana Kelly Silva Maia, Ângela Giovana Batista

13. Guidance for designing a preclinical bioavailability study of bioactive compounds

    Helena Dias de Freitas Queiroz Barros, Cinthia Baú Betim Cazarin, Mario Roberto Maróstica Junior

    Part II: Clinical Trials

14. Protocols related to Nutritional Anamnesis in Head and Neck Cancer Patients

    Judith Büntzel and Jens Büntzel

15. Food Diary, Food Frequency Questionnaire, and 24-hour dietary recall

    Luisa Saravia, Paula Moliterno, Estela Skapino, and Luis A. Moreno

16. The Use of Questionnaires to Measure Appetite

    James H Hollis

17. Protocols for the Use of Indirect Calorimetry in Clinical Research

    Katherine L Ford, Camila LP Oliveira, Stephanie Ramage, and Carla M Prado

18. Anthropometric Assessment Methods for Adults and Older People

    Thalita Cremonesi Pereira and Cinthia Baú Betim Cazarin

19. MRI-based Body Composition Analysis

    Magnus Borga, André Ahlgren, and Sarah Weston

20. Functional tests for assessing human beta-cell function and insulin sensitivity

    Marcelo Miranda de Oliveira Lima and Bruno Geloneze

21. Maximum oxygen consumption - VO2max laboratory assessment

    Mara Patrícia Traina Chacon-Mikahil, Alex Castro, Danilo dos Santos Caruso, and Arthur Fernandes Gáspari

22. 2Muscle and Fat Biopsy and Metabolomics

    Cláudia Regina Cavaglieri, Mara Patrícia Traina Chacon-Mikahil., Renata Garbellini Duft, Ivan Luiz Padilha Bonfante, Arthur Fernandes Gáspari, and Alex Castro