This book constitutes the refereed proceedings of the 25th International Conference on Applications of Evolutionary Computation, EvoApplications 2023, held as part of Evo2023, in April 2023, co-located with the Evo2023 events EuroGP, EvoCOP, and EvoMUSART. The EuroGP focused on the technique of genetic programming, EvoCOP targeted evolutionary computation in combinatorial optimization, and EvoMUSART was dedicated to evolved and bio-inspired music, sound, art, and design.The EvoApplications 2023 presents papers on the different areas: Analysis of Evolutionary Computation Methods: Theory, Empirics, and Real-World Applications, Applications of Bio-inspired Techniques on Social Networks, Evolutionary Computation in Edge, Fog, and Cloud Computing, Evolutionary Computation in Image Analysis, Signal Processing, and Pattern Recognition and others.