------------------------------------------------------------ A handy lab manual that permits quick and easy access to the techniques commonly used to analyse antibody specificity. Some of the most useful immunological techniques based on antibodies are described, including ELISA, immunoblotting and immunoprecipitation protocols that provide useful tools for recognising immunological specificities, and basic immunofluorescence and immunohistochemistry procedures for the in situ identification of antigens. The topics are discussed from a practical point of view, describing the theoretical basis of each technique and sample protocols plus a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing the reader with the maximum possible information on each technique.

Klappentext

A handy lab manual that allows quick and easy access to the techniques commonly used in analysing antibody specificity. It describes some of the most useful immunological techniques based on antibodies, including ELISA, immunoblotting and immunoprecipitation protocols that provide useful tools for recognising immunological specificities, together with basic immunofluorescence and immunohistochemistry procedures for the in situ identification of antigens. The topics are discussed from a practical point of view, combining the theoretical basis of each technique with sample protocols and a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing readers with the maximum possible information on each technique. XXXXXXX NEUER TEXT This handy lab manual permits quick access to the techniques commonly used to analyze antibody specificity. The most useful immunological techniques are described, providing readers with practical tools for recognizing immunological specificities and procedures for the in situ identification of antigens. The theoretical basis of each technique is described and sample protocols and troubleshooting tips are included. A Springer Lab Manual

Inhalt

1 Introduction.- 2 ELISA assays.- 3 Immunoblotting.- 4 Immunoprecipitation.- 5 Immunofluorescence.- 6 Immunohistochemistry.- 7 Antibody Specificity Analyzed by Peptides Synthesized on Cellulose Membranes.- Appendix 1.- Appendix 2.- Appendix 3.