Amino Acid Biosynthesis - Pathways, Regulation and Metabolic Engineering

This book presents the current knowledge of fundamental as well as applied microbiology of amino acids. Coverage details the amino acid biosynthetic pathways, their genetic and biochemical regulation, transport of amino acids and genomics of producing microorganisms. The book also examines the metabolic engineering of microorganisms for the biotechnological production of amino acids for use as pharmaceuticals and as food and feed additives.

Zusammenfassung

Amino Acids Biosynthesis presents the current knowledge of fundamental as well as applied microbiology of amino acids. Topics discussed are the amino acid biosynthetic pathways, their genetic and biochemical regulation, transport of amino acids and genomics of producing microorganisms. The characterization of the control mechanisms of amino acid biosynthesis has revealed insights into principles of genetic and biochemical regulation, such as transcriptional regulators and a new class of regulatory elements, the riboswitch.

The volume further deals with the metabolic engineering of microorganisms for the biotechnological production of amino acids for use as pharmaceuticals and, particularly, as food and feed additives. Comprehensive reviews are given of recent achievements to enable or improve production of amino acids and dipeptides by fermentation and enzyme catalysis. Here, the particular focus is on metabolic engineering, the rational improvement of metabolic functions using recombinant DNA technology.

Inhalt
Production of Glutamate and Glutamate-Related Amino Acids: Molecular Mechanism Analysis and Metabolic Engineering.- The l-Lysine Story: From Metabolic Pathways to Industrial Production.- l-Threonine.- Aromatic Amino Acids.- Branched-Chain Amino Acids.- Methionine Biosynthesis in Escherichia coli and Corynebacterium glutamicum.- Cysteine Metabolism and Its Regulation in Bacteria.- Microbial Arginine Biosynthesis: Pathway, Regulation and Industrial Production.- l-Serine and Glycine.- Alanine, Aspartate, and Asparagine Metabolism in Microorganisms.- Amino Acid Transport Systems in Biotechnologically Relevant Bacteria.- Occurrence, Biosynthesis, and Biotechnological Production of Dipeptides.- Genomes and Genome-Level Engineering of Amino Acid-Producing Bacteria.