This book reviews the latest trends in glycobiotechnology, it offers an authoritative discussion about future directions of glycoengineering, and it provides a comprehensive overview about the current and emerging approaches to identify, quantify and characterize glycosylated proteins.

Divided into 14 chapters, the book outlines recombinant glycoprotein expression in mammalian cells, insect cells, yeast, and bacterial systems. It covers the chemical and enzymatic syntheses of glycans and glyconjugates, and addresses the impact of glycosylation on protein function for the development of biologicals including vaccines. In the final chapters of the book, readers will discover more about the state-of-the-art in glycomics, glycoproteomics and glycan array technologies.

Autorentext

Dr. Erdmann Rapp is heading the Bio/Process Analytics team of the Bioprocess Engineering Department at the Max Planck Institute for Dynamics of Complex Technical Systems in Magdeburg and founder, CEO & CSO of glyXera, providing tailored cutting-edge glycoanalytical products and services. He is an experienced researcher, inventor, mentor and university lecturer. He and his team are working on the development and implementation of innovative bio/process analytics - in particular, on sophisticated tools for proteomics, glycoproteomics and glycomics - for a deeper understanding of biological and biotechnological processes. Dr. Rapp's successful research is documented by more than 120 peer-reviewed papers and book chapters, and 8 patents.Professor Udo Reichl is a Scientific Member of the Max Planck Society, Director of the Bioprocess Engineering Department of the Max Planck Institute for Dynamics of Complex Technical Systems in Magdeburg and Chair of Bioprocess Engineering at the Otto-von-Guericke University Magdeburg. He has profound expertise in the development and optimization of cell culture-based viral vaccine production. His group developed sophisticated analytical tools to monitor process dynamics, metabolism of cell lines, infection dynamics and the glycosylation of viral and recombinant proteins. His list of publications comprises more than 250 peer-reviewed papers and book chapters, and he has filed various patents.

Inhalt

Glyco-engineering of mammalian expression systems on process level.- Glyco-engineering of mammalian expression systems on cellular level.- Glyco-engineering of insect cell expression systems.- Glyco-engineering of yeast expression systems.- Glyco-engineering of plant(cell) expression systems.- Glyco-engineering of bacteria expression systems.- Chemical synthesis of glycans and glycoconjugates.- Enzymatic synthesis of glycans and glycoconjugates.- The interplay/impact of glycosylation and/on protein function for recombinant proteins and mAbs.- The interplay/impact of glycosylation and/on protein function for recombinant proteins and mAbs.- The interplay/impact of glycosylation and/on protein function for bacterial vaccines.- Glycomicstechnologies.- Glycoproteomicstechnologies.- Glycan array technologies.