Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse

Inhalt
Inhalteverzeichnis. Contents..- Peptides (Bound Amino Acids) and Free Amino Acids.- A. Peptides (Bound Amino Acids).- I. Higher Plants.- II. Algae.- III. Fungi.- IV. Glutathione.- V. Pteroic Acid Derivatives etc..- B. Amino Acids.- I. Amino Acids Found in Plants.- II. Determination of Free Amino Acids: General Methods.- III. Special Methods for Free Amino Acids.- IV. Some Results of Analyses for Free Amino Acids in Plant Materials.- References.- Proteins.- A. Methods for Separating Protein from Non-Protein Nitrogenous Material.- B. Methods for Determining Protein.- 1. Determination of Total Dry Matter.- 2. Determination of Nitrogen.- 3. Determination of Amino Acids.- 4. Determination of the Peptide Bond.- 5. Phydcal Methods.- 6. Methods Depending on Specific Activities.- C. The Amino Acid Composition of Tissues and Isolated Proteins.- D. Leaf Proteins.- I. The Normal Mature Leaf.- II. The Proteins of Virus Infected Leaves.- III. The Immature Leaf and Other Young Tissues.- E. Proteinsfrom Miscellaneous Parts of the Plant.- 1. Flowers.- 2. Fruits.- 3. Pollen.- 4. Proteins in Latex.- 5. Proteins in Roots, Tubers and Other Underground Parts of the Plant.- F. Proteins in Algae.- References.- Seed Proteins.- A. Some General Considerations.- I. The Major Seed Proteins.- II. Other Seed Proteins.- 1. Enzymes.- 2. Inhibitors and Toxic Proteins.- III. Amino Acid Composition.- B. Procedures.- I. Choice and Preparation of Initial Material.- II. Extraction.- C. Experimental Procedures for the Preparation of Different Types of Protein Fractions from Seeds.- I. Globulin Fractions.- II. Prolamine from a Cereal Grain.- III. Enzymes, Inhibitors and Toxic Proteins.- References.- Methods of Determining the Nutritive Value of Proteins.- A. Evaluation for Ruminants.- B. Evaluation for Non-Ruminants.- I. Techniques Involving Chemical Analysis, or Microbiological or Enzymatic Assay.- II. Techniques Involving the Use of Animals.- 1. Simple Substitution Technique.- 2. Succesive Substitution Technique, with Nitrogen Balance Trials.- 3. Gross Protein Value.- 4. Biological Value of Protein.- 5. Net Protein Value.- 6. Protein Efficiency Ratio.- 7. Carcase Analysis Technique.- 8. Protein Repletion Techniques.- References.- Urea and Ureides.- A. The Occurence of Urea and its Precursors in Plants.- I. Intermediates in Purine Catabolism.- 1. Uric Acid and Uricase.- 2. Allantoin and Allantoinase.- 3. Allantoic Acid and Allantoicase.- 4. Urea and Urease.- II. Arginine as a Urea Precursor.- III. Canavanine as a Urea Precursor.- IV. Citrulline and Hydantoin.- V. Thiourea.- B. Some Analytical Principles.- I. Uric Acid.- II. Allantoin and Allantoic Acid.- III. Urea.- IV. Arginine, Canavanine, Citrulline and other Compounds.- References.- Chlorophylls: Analysis in Plant Materials.- A. Chlorophylls a and b.- I. Structure and Distribution.- II. Preparation.- III. Physical Properties of Chlorophylls a and b.- IV. Qualitative Test for Chlorophylls a and b.- V. Tests for Purity.- VI. Pheophytins a and b.- VII. Quantitative Determination of Chlorophylls a and b.- B. Chlorophyll c.- I. Preparation of Chlorophyll c.- II. Physical Properties of Chlorophyll c.- III. Qualitative Tests.- IV. Pheophytin c.- V. Quantitative Determination of Chlorophyll c.- C. Chlorophyll d.- I. Preparation of Chlorophyll d.- II. Physical Properties of Chlorophyll d.- III. Qualitative Tests.- IV. Pheophytin d.- V. Quantitative Determination of Chlorophyll d.- D. Chlorophyll e.- E. Protochlorophyll.- I. Preparation of Protochlorophyll.- II. Physical Properties of Protochlorophyll.- III. Analytical Tests.- IV. Protopheophytin.- V. Quantitative Determination of Protochlorophyll.- F. Bacteriochlorophyll.- I. Preparation of Bacteriochlorophyll.- II. Physical Properties of Bacteriochlorophyll.- III. Qualitative Tests.- IV. Bacteriopheophytin.- V. Quantitative Determination of Bacteriochlorophyll.- G. Bacterioviridin.- I. Preparation of Bacterioviridin.- II. Physical Properties of Bacterioviridin.- III.Qualitative Tests.- IV. Bacterioviridin Pheophytin.- H. Special Topics.- I. Preparatory Procedures.- II. Spectrophotometry.- III. Chromatography.- IV. Miscellaneous Procedures.- V. Chlorophyllase.- References.- Haematin Compounds.- A. Spectroscopic Methods.- I. Introduction.- II. Spectroscopes for Analytical Purposes.- III. Apparatus for Comparison Spectroscopy.- IV. Pupillen-Spektroskop.- V. A Comparison of the Microspectroscope and Pupillen-Spektroskop.- VI. The Spectrocolorimeter.- VII. Hartridge Reversion Spectroscope.- VIII. Intensification of Absorption Spectra at Low Temperatures.- IX. Detection of Haematin in Plant Tissues.- X. Estimation of Total Haematin.- B. Cytochromes.- I. Spectral Characteristics.- II. Types of Cytochrome Spectra in Plant Materials.- 1. Cytochromes in Tissues Devoid of Chlorophyll.- 2. Cytochromes in Leaves.- 3. Cytochrome in Marine Algae.- 4. Soluble Cytochrome Components.- III. Quantitative Estimation of Cytochromes.- IV. Cytochrome Oxidase.- C. Haematin Enzymes: Peroxidase and Catalase.- I. Peroxidase.- II. Catalase.- III. Spectroscopy of Peroxidase and Catalase.- D. Root Nodule Haemoglobin.- References.- Nucleic Acids, their Components and Related Compounds.- A. Occurence in Plants.- B. Nucleic Acids: General Structure and Composition.- C. Absorption Spectra.- I. Nucleic Acids.- II. The Smaller Constituents.- D. The Reactions and the Identification of the Purines and Pyrimidines, their Ribosides and Nucleotides.- I. The Purines: General Reactions.- II. The Pyrimidines.- III. The Nucleosides.- IV. The Nucleotides.- E. Paper Chromatography.- I. General Considerations.- II. The Detection of Nucleic Acid Derivatives on Chromatograms.- III. The Separation and Identification of Purines, Pyrimidines and Nucleosides.- F. The Quantitative Analysis of Nucleic Acids for their Bases.- I. Ribonucleic Acids.- II. The Quantitative Analysis of DNA.- G. Separation and Identification of Nucleosides and Nucleotides.- I. Paper Chromatography.- II. Methods for the Identification of the Isomeric Nucleotides.- III. Ion Exchange Chromatography.- IV. The Separation of Nucleic Acid Derivatives by Electrophoresis on Paper.- H. Some Enzymes Used in Nucleic Acid Chemistry.- I. Determination of Phosphate and Carbohydrate.- I. The Analysis of Phosphorus.- II. The Diphenylamine Reaction for Deoxypentose.- III. The Estimation of Pentoses.- J. Other Methods for the Analysis and Determination of Nucleic Acids.- I. Analysis of Ribonucleic Acids by Means of Silver Precipitation.- II. The Recognition of the Nucleic Acids in Tissues by Microscopical Methods.- III. The Estimation of Nucleic Acids in Tissues.- K. The Isolation of Nucleic Acids from Plant Tissues.- I. The Isolation of Deoxyribonucleic Acid from Wheat Germ.- II. Isolation of Ribonucleic Acids from Plant Tissues.- L. Other Purines and Pyrimidines in Plants.- I. Vicine and Divicine.- II. The Methylated Xanthine Derivatives (Caffein and Theobromine).- References.- Adenosine Diphosphate, Adenosine Triphosphate.- A. Occurence and Distribution.- B. Isolation of Adenosine Triphosphate.- I. Difficulties in Isolation.- II. Criteria for Purity.- III. The Isolation of Adenosine Triphosphate from Mung Beans (Phasedus aureus).- C. Determination of Adenosine Triphosphate.- I. Enzymatic Determination.- II. Identification of ATP by Paper Chromatography.- III. The Quantitative Estimation of ATP by Column Chromatography.- D. The Status of Adenosine Diphosphate.- References.- Codehydrasen I und II (Diphospho-pyridin-nucleotid und Triphospho-pyridin-nucleotid).- A. Einleitung.- B. Analytische Verfahren zur Bestimmung der Codehydrasen.- I. Allgemeines.- 1. Der Zustand der Codehydrasen im pflanzlichen Material u. ihre Extraktion.- 2. Bestimmung von Diphospho-pyridin-nucleotid.- 3. Bestimmung von Triphospho-pyridin-nucleotid.- II. Die Thunberg-Technik.- III. Manometrische Methoden.- IV. Spektrophotometrische Methoden.- C. Darstellung von Codehydrasen.- 1. Darstellung von Diphosph…