Culture of Animal Cells

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made - particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.

This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.

Auteur

R. IAN FRESHNEY, PhD, is an honorary Senior Research Fellow at the Instituteof Cancer Sciences at the University of Glasgow in Glasgow, UK. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.

Contenu
List of Figures, xix

List of Color Plates, xxiii

List of Tables, xxv

List of Protocols, xxvii

List of Minireviews, xxix

Preface and Acknlowledgments, xxxi

Abbreviations, xxxiii

About the Companion Website xxxviii

1. Introduction, 1

2. Biology of Cultured Cells, 19

3. Laboratory Design and Layout, 37

4. Equipment and Materials, 51

5. Aseptic Technique, 73

6. Safety, Bioethics, and Validation, 89

7. Culture Vessels and Substrates, 111

8. Defined Media and Supplements, 125

9. Serum-Free Media, 149

10. Preparation and Sterilization, 173

11. Supplementary Material*

12. Primary Culture, 207

13. Supplementary Material*

14. Subculture and Cell Lines, 235

15. Authentication and Validation, 259

16. Microbial Contamination, 289

17. Cryopreservation and Banking, 307

18. Cloning and Selection, 327

19. Supplementary Material*

20. Cell Sorting, 347

21. Cell Line Characterization, 359

22. Differentiation, 383

23. Three?]Dimensional Culture 401

24. Scale?]up and Automation, 427

25. Senescence, Immortalization, and Transformation, 455

26. Quantitation, 483

23 Supplementary Material*

24. Cytotoxicity, 513

25. Supplementary Material*

26. Culture of Specific Cell Types, 531

27. Supplementary Material*

28. Stem Cells, 571

29. Supplementary Material*

30. Training Programs, 591

31. Problem Solving 627

32. In Conclusion, 651

Supplementary Material*

Appendix III: Sources of Equipment and Material

Appendix IV: Suppliers and Other Resource