Methods in Protein Biochemistry

This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.

Auteur

Harald Tschesche, University of Bielefeld, Germany.

Texte du rabat

This book presents a survey of recent developments in protein biochemistry. As new analytical methods and techniques become available to the protein chemist for isolating, purifying, analyzing, and characterizing novel proteins, new insights into basic and applied biochemistry are made possible. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones, or the folding of membrane proteins in detergents, or of the control of glycoprotein folding in the endoplasmic reticulum. Methods are described to follow dynamic changes in conformation and folding processes, or the protein import into the intermembrane space. Direct on membrane identification of gel-resolved proteins by Maldi mass spectrometry allows proteome analysis after D-DIGE and discovery of biomarkers in pathophysiological conditions (Chronic Obstructive Pulmonary Disease, Duchenne Muscular Dystrophy). Mass spectrometry determines protein complexes after chemical cross-linking and small molecule binding sites by Capture Compound MS. Correlated single-crystal spectroscopy and synchrotron X-ray crystallography are described as well as wide-angle X-ray solution scattering (WAXS) of proteins to generate information about structure, flexibility and dynamics. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The chemical modification in living cells has become possible and also probing of protein dynamics in vivo. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.

• Compact reviews of new methods and techniques in protein chemistry and biochemistry
• Numerous practical examples
• In textboxes valuable advices to avoid fundamental mistakes
• Full colour layout

Contenu

• Membrane protein folding in detergents, Deber
• Three phase partitioning (TPP), Dennison
• Protein import into the intermembrane space of mitochondria, Herrmann
• A novel PVDF membrane for on-membrane identification of gel-resolved proteins by MALDI-MS, Hirano
• Molecular mechanisms of chaperone action, Hoehfeld
• Proteomics of Duchenne muscle dystrophy, Kamp
• Analysis of protein complexes using chemical cross-linking and mass spectrometry, Leitner
• Determining the small molecule binding site of a protein by Capture Compound Mass Spectrometry (CCMS), Lenz
• Pathobiology of antitrypsin defiency and the serpinopathies, Lomas
• Wide-angle x-ray solution scattering method (WAXS), Makowski
• Bioaffinity-mass spectrometry: Molecular approaches and applications protein biochemistry, Przybylski
• Laser ablation electrospray ionization mass spectrometry (LAESI), Razunguzwa
• Design and applications of protein epitope mimetics, Robinson
• Probing protein dynamics in vivo: bacterial acyl carrier protein as a case study, Volkmann
• Target oriented peptide-arrays in a palliative approach to mucoviszidose, Volkmer
• Correlated single-crystal spectroscopy and x-ray crystallography, Orville
• Chemical modification of proteins in the living cell, Correa Jr.
• Relevance of peptide purity for the reproducibility of fibril formation, Glockshuber
• Proteome analysis of humen BAL by two-dimensional difference in gel-Electrophoresis (D-DIGE): COPD patients vs control subjects, Mileo, Beranova-Giorgianni, Giorgianni
• Dynamics of changes in conformation and folding processes in peptides and proteins, Kiefhaber, Reiner
• Glykoprotein folding within the endoplasmic reticulum, Parodi