Vascular Proteomics

This book details methods and protocols for the analysis of arteries, cells, lipoproteins, body fluids, and metabolites, with a particular focus on MS-based methods of protein and peptide quantification. Includes step-by-step, readily reproducible protocols.

Proteomics is a rapidly expanding investigation platform in cardiovascular medicine. Driven by major improvements in mass spectrometry (MS) instrumentation and data analysis, the proteomics field has flourished in recent years particularly in the study of complex diseases. These recent advances are characterized by the development of quantitative MS-based methods that promoted the field from primarily identifying proteins to also providing measurements of relative changes in protein levels between different cell states. This progress is reflected in the application of proteomic techniques to vascular pathology. Vascular Proteomics: Methods and Protocols provides up-to-date methods and protocols for the analysis of arteries, cells, lipoproteins, body fluids, and metabolites, with a particular focus on MS-based methods of protein and peptide quantification. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Vascular Proteomics: Methods and Protocols is a representative selection of methods that can be a useful resource for experienced proteomics practitioners as well as newcomers interested in becoming acquainted with the practice of proteomic techniques for cardiovascular research.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Contenu

Vascular Proteomics.- Laser Microdissection and Saturation Labeling Dige Method for the Analysis of Human Arteries.- Use of TOF-SIMS in Vascular Biology.- Proteomic Analysis of Vascular Smooth Muscle Cells with S- and R-Enantiomers of Atenolol by ITRAQ and LC-MS/MS.- Phosphoproteomic Analysis of Aortic Endothelial Cells Activated by Oxidized Phospholipids.- Characterization of Membrane and Cytosolic Proteins of Erythrocytes.- Characterization and Analysis of Human Arterial Tissue Secretome by 2-DE and NLC-MS/MS.- Identification of Novel Biomarkers of Abdominal Aortic Aneurysms (AAA) by 2D-DIGE and MALDI-MS FROM AAA-Thrombus Conditioned Media.- Metabolites Secreted by Human Atherothrombotic Aneurysm.- Quantitative Analysis of Apolipoproteins in Human HDL by Top-Down Differential Mass Spectrometry.- Quantitative Proteomics Analysis of High-Density Lipoproteins by Stable 18O-Isotope Labeling.- Unraveling Biomarkers of Abdominal Aortic Aneurisms by iTRAQ Analysis of Depleted Plasma.- Absolute Quantitation of Proteins in Human Blood by Multiplexed Multiple Reaction Monitoring Mass Spectrometry.- Multiple Reaction Monitoring (MRM) of Plasma Proteins in Cardiovascular Proteomics.- Proteomic Analysis of Plasma of Patients with Left Ventricular Remodeling After Myocardial Infarction. Usefulness of SELDI-TOF.- Proteomic Analysis of Urinary Exosomes in Cardiovascular and Associated Kidney Diseases by Two Dimensional Electrophoresis and LC-MS/MS.