Tissue Engineering, Stem Cells, and Gene Therapies

Recent years have seen an upsurge of significant interest in cell-based technologies. A range of productive and lively debate have taken place relating to tissue engineering, namely the construction of tissues and whole organs using molecularly-designed resorbable biomaterials to create tissue de novo, the potential use of human embryonic stem cells for transplantation and regenerative medicine, with similar potential for adult-derived stem cells, and gene therapy, in relation to cell transplantation. New findings in biomimetic materials, cell signalling pathways, extracellular matrix receptors and ligands, growth factors, and the human genome project, all present particularly motivating sources for the development of research in the evergrowing biomedical field. The purpose of this book is to stimulate further the work in biomedicine and to make the issues of related scientific disciplines accessible to a wider readership by characterising the current state of research in the biomedical field. The lectures and a selection of the presentations from BlOMED 200- th The 9 International Symposium on Biomedical Science and Technology, held in September 2002 in Turkey- constitute the basis for the volume. Tissue engineering, stem cells, cell and gene therapies were the major topics presented and discussed in the symposium. This book is intended to serve as an up-to-date synopsis of the major developments of our area through the work reflected in BlOMED 2002, though not covering all aspects of the topics, due to the natural restrictions within a volume of this kind.

Includes manuscripts on tissue engineering, stem cells and gene therapies authored by world-renowned scientists of the field

Texte du rabat

The first section of the volume consists of four chapters giving perspectives for the current status and potential future of tissue engineering and stem cell technologies.

The second section of the volume includes five chapters based on experimental and clinical data. In this section, the role of stem cells in liver tissue engineering, cell-based therapies in diabetes mellitus, and chronic degenerative diseases of the central nervous system, and adult-derived stem cell therapies are discussed.

The two chapters of the third section focus on the biomarkers for tissue-engineered products, namely for tissue-engineered skin.

The eight chapters of the fourth section discuss novel biomaterials developed for neural-, vascular-, aortic-, bone-, cartilage- and endocrine pancreas-tissue engineering applications.

The last section of the book includes chapters on practical gene targeting applications, controlled release in gene therapy and tissue engineering, antibodies in cancer, acute-phase genes and phage-displayed peptide libraries.

Contenu
I. Perspectives for Tissue Engineering and Stem Cells.- 1. Tissue engineering: confronting the transplantation crisis.- 2. Human embryonic stem cells-realising the potential.- 3. Human embryonic or adult stem cells: an overview on ethics and perspectives for tissue engineering.- 4. Stem cell biology and plasticity.- II. Stem Cells in Tissue Engineering and Cell - Based Therapies.- 5. Liver tissue engineering: successes and limitations.- 6. Pancreatic islet and stem cell transplantation in diabetes mellitus: results and perspectives.- 7. Cell based therapy of chronic degenerative diseases of the central nervous system.- 8. CD 34+ cells in hematopoietic stem cell transplantation.- 9. The effects of different growth factors on human bone marrow stromal cells differentiating into hepatocyte-like cells.- III. Biomarkers for Tissue-Engineered Products.- 10. Oxidative DNA damage biomarkers used in tissue-engineered skin.- 11. Biomarkers used to detect genetic damage in tissue-engineered skin.- IV. Biomaterials in Tissue Engineering and Repair.- 12. Design peptide scaffolds for regenerative medicine.- 13. Progresses in synthetic vascular prostheses: toward the endothelialization.- 14. Adhesion and growth of rat aortic smooth muscle cells on lactide-based polymers.- 15. Biodegradable copolymers carrying cell-adhesion peptide sequences.- 16. Polymer based scaffolds and carriers for bioactive agents from different natural origin materials.- 17. Preparation and characterization of natural/synthetic hybrid scaffolds.- 18. Polyhipe polymer: a novel scaffold for in vitro bone tissue engineering.- 19. Pancreatic islet culture and transplantation using chitosan and PLGA scaffolds.- V. Gene Therapy and Other Approaches.- 20. Challenges and prospects for targeted transgenesis in livestock.- 21. Controlled release of bioactive agents in gene therapy and tissue engineering.- 22. Interleukin 1 (IL-1) induces the activation of stat3.- 23. The use of antibodies in diagnosis and therapy of cancer.- 24. Detection of phage displayed peptides with blocking ability in vascular endothelial growth factor (VEGF) model.- 25. Index.