Single Cell Metabolism

This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Contenu
Single Cell Metabolomics by Mass Spectrometry.- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry.- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry.- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis.- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells.- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics.- Laser Capture Microdissection Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells.- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging.- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population.- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry.- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry.- Compact Quantum Dots for Quantitative Cytology.- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry.- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry.- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics.- Ten Major Future Challenges in Single Cell Metabolomics.