Methods in Membrane Lipids

Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. This volume provides a comprehensive set of techniques for studying membrane lipids, and their interactions with other membrane lipids, with membrane proteins, and with exogenous agents such as anesthetics. The progression of chapters increases in complexity, initially presenting introductory concepts and methods for the study of simple structures and interactions, then moving to the study of more complex structures and interactions, including: behaviors of lipids in water systems and phases; membrane lipid movements and diffusion; properties of lipids in mono and bilayers; complex lipid domains; and membrane-lipid interaction with proteins and drugs. Each chapter is written by an internationally recognized expert in the field.

A comprehensive set of methods for studying membrane lipids with a strong biophysical emphasis Provides comparison of various available techniques including pros and cons as seen by the experts Each chapter includes an introduction to the particular topic and method

Contenu
A Glance at the Structural and Functional Diversity of Membrane Lipids.- Membrane Lipid Polymorphism.- Acrylodan-Labeled Intestinal Fatty Acid-Binding Protein to Measure Concentrations of Unbound Fatty Acids.- Measuring Molecular Order and Orientation Using Coherent Anti-Stokes Raman Scattering Microscopy.- Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scattering.- Nuclear Magnetic Resonance Investigation of Oriented Lipid Membranes.- Molecular Dynamics Simulations as a Complement to Nuclear Magnetic Resonance and X-Ray Diffraction Measurements.- Use of Inverse Theory Algorithms in the Analysis of Biomembrane NMR Data.- Statistical Thermodynamics Through Computer Simulation to Characterize Phospholipid Interactions in Membranes.- Fluorometric Assay for Detection of Sterol Oxidation in Liposomal Membranes.- Fluorescence Detection of Signs of Sterol Superlattice Formation in Lipid Membranes.- Differential Scanning Calorimetry in the Study of Lipid Phase Transitions in Model and Biological Membranes.- Pressure Perturbation Calorimetry.- Fourier Transform Infrared Spectroscopy in the Study of Lipid Phase Transitions in Model and Biological Membranes.- Optical Dynamometry to Study Phase Transitions in Lipid Membranes.- Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes.- Measurement of Lateral Diffusion Rates in Membranes by Pulsed Magnetic Field Gradient, Magic Angle Spinning-Proton Nuclear Magnetic Resonance.- Using Fluorescence Recovery After Photobleaching to Measure Lipid Diffusion in Membranes.- Single-Molecule Fluorescence Microscopy to Determine Phospholipid Lateral Diffusion.- Modeling 2D and 3D Diffusion.- Measurement of Water and Solute Permeability by Stopped-Flow Fluorimetry.- Fluorescence Microscopy to Study Pressure Between Lipids in Giant Unilamellar Vesicles.- X-Ray Scattering and Solid-State Deuterium Nuclear Magnetic Resonance Probes of Structural Fluctuations in Lipid Membranes.- Determination of Lipid Spontaneous Curvature From X-Ray Examinations of Inverted Hexagonal Phases.- Shape Analysis of Giant Vesicles With Fluid Phase Coexistence by Laser Scanning Microscopy to Determine Curvature, Bending Elasticity, and Line Tension.- Laser Tweezer Deformation of Giant Unilamellar Vesicles.- Measurement of Lipid Forces by X-Ray Diffraction and Osmotic Stress.- Micropipet Aspiration for Measuring Elastic Properties of Lipid Bilayers.- Langmuir Films to Determine Lateral Surface Pressure on Lipid Segregation.- Detergent and Detergent-Free Methods to Define Lipid Rafts and Caveolae.- Near-Field Scanning Optical Microscopy to Identify Membrane Microdomains.- Fluorescence Microscopy to Study Domains in Supported Lipid Bilayers.- Fluorescence Resonance Energy Transfer to Characterize Cholesterol-Induced Domains.- Lipid Domains in Supported Lipid Bilayer for Atomic Force Microscopy.- Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers.- Laurdan Studies of Membrane Lipid-Nicotinic Acetylcholine Receptor Protein Interactions.- Single-Molecule Methods for Monitoring Changes in Bilayer Elastic Properties.- Ion-Channel Reconstitution.- The Use of Differential Scanning Calorimetry to Study Drug-Membrane Interactions.- Atomic Force Microscopy to Study Interacting Forces in Phospholipid Bilayers Containing General Anesthetics.