Glycobiology

Klappentext In the past decade, a tremendous progress in understanding the function of carbohydrates or glycans took place. This progress also starts showing the promise of drug development based on the function of carbohydrates. This critical progress largely owes a development of new methodology to determine a role of a carbohydrate and then develop a method to interfere such biological process, mostly taking a form of protein-carbohydrate interaction. In the current three volumes of Methods in Enzymology focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers own research efforts. Each chapter has been designed so that enough scientific background will be given in each chapter for further development of methods by readers themselves. The books thus should be useful for all levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors and to all levels of scientists in research institutes including industry. The editor is highly hopeful and confident that these volumes of Methods in Enzymology will contribute to another surge in determining the structure and function of glycans and developing new drugs based on carbohydrate functions. Zusammenfassung A collection focusing on glycomics. It lets readers appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts. It is suitable for different levels of scientists starting from the last years of colleges! graduate students! postdoctoral fellows to professors. Inhaltsverzeichnis Section I. Proteoglycans and Sulfotransferases 1. 3-O-sulfotransferases Nicholas Scworak 2. Proteoglycan and signal transduction Michael Simon 3. Glypican function Xinhua Lin 4. Glycomics profiling of heparan sulfate Jeremy Turnbull Section II. Infection and lectin 5. Recognition of carbohydrates by natural killer T cells Mitch Kronenberg 6. Microbial glycoconjugates in innate immunology Antonio Molinaro 7. Schistosome glycolipids and their role in innate immunity Irma Van Die and Rudolf Guyer 8. Carbohydrate-specific signaling through the DC-SIGN Teunis Geijtenbeek 9. Natural and engineered carbohydrate-recognition domains for targeted glycoproteomic analysis of cell surface glycosylation and identification of ligands for glycan-binding receptors Kurt Drickamer 10. MRH-domain containing lectin ERAD Nobuko Hirosawa 11. Galectin and the control of immune tolerance Gabriel Rabinovich 12. Galectin and cell signaling Michel Demetriou 13. Galectin-1 and HIV infection Sachiko Sato Secion III. Drosophila 14. Drosophila N- and O-glycans Michael Tiemeyer 15. MRH-domain containing lectin ERAD Nobuko Hirosawa 16. Drosophila proteoglycans Shoko Nishihara Section IV. Notch Signaling 17. O-GlcNAc modification of EGF domain of notch Tetsuya Okajima 18. Regulation of Notch signaling via O-glycosylation Jafar-Nejad Hamed Section V. New Development 19. O-fucosylation of Thrombospondin type 1 repeats Robert Haltiwanger 20. Unique glycans attached to unique glycoproteins Michael Pierce 21. Autoantibodies against carbohydrates Michel Hollingsworth 22. GPRH is critical for Golgi apparatus function Yusuke Maeda and Taroh Kinoshita 23. Genome-wide RNAi screen for N-glycosylation loci Weston B Struwe 24. Fucosyl glycans that bind to Helicobacter pylori Tatsuo Miyazaki and Katsumi Ajisaka 25. Seven-pass transmembrane glycoprotein, a glucose-responding receptor Yoshio Hirabayashi ...

Résumé
A collection focusing on glycomics. It lets readers appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts. It is suitable for different levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors.

Contenu

Section I. Proteoglycans and Sulfotransferases

1. 3-O-sulfotransferases

Nicholas Scworak

2. Proteoglycan and signal transduction

Michael Simon

3. Glypican function

Xinhua Lin

4. Glycomics profiling of heparan sulfate

Jeremy Turnbull

Section II. Infection and lectin

5. Recognition of carbohydrates by natural killer T cells

Mitch Kronenberg

6. Microbial glycoconjugates in innate immunology

Antonio Molinaro

7. Schistosome glycolipids and their role in innate immunity

Irma Van Die and Rudolf Guyer

8. Carbohydrate-specific signaling through the DC-SIGN

Teunis Geijtenbeek

9. Natural and engineered carbohydrate-recognition domains for targeted glycoproteomic analysis of cell surface glycosylation and identification of ligands for glycan-binding receptors

Kurt Drickamer

10. MRH-domain containing lectin ERAD

Nobuko Hirosawa

11. Galectin and the control of immune tolerance

Gabriel Rabinovich

12. Galectin and cell signaling

Michel Demetriou

13. Galectin-1 and HIV infection

Sachiko Sato

Secion III. Drosophila

14. Drosophila N- and O-glycans

Michael Tiemeyer

15. MRH-domain containing lectin ERAD

Nobuko Hirosawa

16. Drosophila proteoglycans

Shoko Nishihara

Section IV. Notch Signaling

17. O-GlcNAc modification of EGF domain of notch

Tetsuya Okajima

18. Regulation of Notch signaling via O-glycosylation

Jafar-Nejad Hamed

Section V. New Development

19. O-fucosylation of Thrombospondin type 1 repeats

Robert Haltiwanger

20. Unique glycans attached to unique glycoproteins

Michael Pierce

21. Autoantibodies against carbohydrates

Michel Hollingsworth

22. GPRH is critical for Golgi apparatus function

Yusuke Maeda and Taroh Kinoshita

23. Genome-wide RNAi screen for N-glycosylation loci

Weston B Struwe

24. Fucosyl glycans that bind to Helicobacter pylori

Tatsuo Miyazaki and Katsumi Ajisaka

25. Seven-pass transmembrane glycoprotein, a glucose-responding receptor

Yoshio Hirabayashi