Hematological Malignancies

This Methods in Molecular Biology(TM) book explores molecular-based assays commonly used in the diagnostic hematopathology and laboratory hematology. Includes step-by-step, readily reproducible protocols, materials lists, trouble shooting tips and more.

Increased knowledge on the pathogenesis of hematologic diseases has been translated into diagnostic and prognostic applications. Hematopathology and laboratory hematology were among the first disciplines to embrace molecular diagnostics. Hematological Malignancies:Methods and Protocols, explores molecular-based assays frequently used in the routine diagnostic hematopathology and laboratory hematology. Many of these protocols were initially developed as research applications and were further refined as they transitioned to the diagnostic laboratory. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Hematological Malignancies: Methods and Protocols aids scientist in the continuing study of tests essential for contemporary laboratory diagnostics of hematological neoplasms.

Features tips on trouble shooting and avoiding known pitfalls Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Contenu

Quantitative BCR-ABL1 RQ-PCR Fusion Transcript Monitoring in Chronic Myelogenous Leukemia.- Detection of BCR-ABL1 Kinase Domain Mutations Causing Imatinib Resistance in Chronic Myelogenous Leukemia.- Laboratory Detection of JAK2 V617F in Human Myeloproliferative Neoplasms.- c-kit Mutational Analysis in Paraffin Material.- Detection of Recurrent Cytogenetic Abnormalities in Acute Lymphoblastic and Myeloid Leukemias using Fluorescence In Situ Hybridization.- Liquid Bead Array Technology in the Detection of Common Translocations in Acute andChronic Leukemias.- Molecular Genetic Tests for FLT3 , NPM1 , and CEBPA in Acute Myeloid Leukemia.- Flow Cytometric Monitoring of Residual Disease in Acute Leukemia.- Chimerism Analysis Following Hematopoietic Stem Cell Transplantation.- Detection of Clonal Immunoglobulin Heavy Chain Gene Rearrangements by the Polymerase Chain Reaction and Capillary Gel Electrophoresis.- Detection of Clonal T-cell Receptor Beta and Gamma Chain Gene Rearrangement by Polymerase Chain Reaction and Capillary Gel Electrophoresis.- Detection of Genetic Translocations in Lymphoma using Fluorescence in situ Hybridization.- Molecular Detection of t(14;18)(q32;q21) in Follicular Lymphoma.- Detection of t(11;14)(q13;q32) in Mantle Cell Lymphoma by Polymerase Chain Reaction.- Detection of t(2;5)(p23;q35) in Anaplastic Large Cell Lymphoma by Long-range Nested Polymerase Chain Reaction Assay.- EBER In Situ Hybridization for Epstein-Barr Virus.-Epstein-Barr virus (EBV) Load Determination Using Real-time Quantitative Polymerase Chain Reaction.- Molecular and Immunohistochemical Detection of Kaposi Sarcoma Herpesvirus / Human Herpesvirus-8.- Detection of Cytomegalovirus Infection by Quantitative Polymerase Chain Reaction.- Thiopurine S-Methyltransferase Pharmacogenetics in Childhood Acute Lymphoblastic Leukemia.- MicroRNA Expression Profiling Using Microarrays