15N Tracing of Microbial Assimilation, Partitioning and Transport of Fertilisers in Grassland Soils

This book presents innovative research on soil nitrogen cycling and nitrate leaching with a view to improving soil management and fertiliser nitrogen use efficiency and reducing nitrogen leaching losses. In this regard, nitrogen-15 (15N)-labelled fertiliser was used as a biochemical and physical stable isotope tracer in laboratory and field experiments. The major outcome of the research was the development, validation and application of a new compound-specific amino acid 15N stable isotope probing method for assessing the assimilation of fertiliser nitrogen by soil microbial biomass. The novelty of the method lies in its tracing of incorporated nitrogen into newly biosynthesised microbial protein in time-course experiments using gas chromatography-combustion-isotope ratio mass spectrometry. The approach provides previously unattainable insights into the microbial processing of different nitrogen fertilisers in different soils. Further, it identifies the mechanistic link between molecular-scale processes and observations of field-scale fertiliser nitrogen immobilisation studies. The method and the results presented here will have far-reaching implications for the development of enhanced recommendations concerning farm-based soil management practices for increasing soil productivity and reducing nitrogen losses, which is essential to minimising environmental impacts.

Nominated as an outstanding PhD thesis by the University of Bristol, UK Presents an extremely thorough and well-referenced introduction to sustainable soil nitrogen management Provides detailed information on the compound-specific 15N amino acid stable isotope probing method Describes the first experimental demonstrations of differential processing of N-fertilisers using soil microbes

Auteur
Alice received her B.Sc. in Chemistry from the University of Bristol. In the latter years of her degree she became fascinated by how organic chemistry and stable isotopes could be combined to probe environmental processes. This led her to pursue a Ph.D., in collaboration with Wessex Water, the goal of which was to help mitigate nitrogen fertiliser pollution problems in drinking water supplies. After completing her Ph.D., Alice pursued postdoctoral research at Rothamsted Research, North Wyke, where she was part of a NERC-funded project investigating nitrogen cycling and gaseous nitrogen losses from sheep urine patches on upland soils.

Contenu

Introduction.- Sites, sampling, materials and methods.- Compound-specific amino acid 15N stable isotope probing of nitrogen assimilation by the soil microbial biomass using gas chromatography-combustion- isotope ratio mass spectrometry.- Biosynthetic routing, rates and extents of microbial fertiliser nitrogen assimilation in two grazed grassland soils.- Microbial fertiliser nitrogen assimilation in the field as compared with the laboratory incubation experiments.- 15N tracing of the partitioning and transport of nitrate under field conditions.- Overview and future work.