Experimental Techniques in Molecular Biology

Autorentext

Akhtar Ali has been a professor at the University of Tulsa since 2007. He teaches Experimental Techniques in Molecular Biology, Introduction to Virology, Biotechnology and Genetic Engineering and Biology of Cancer. In 2017, Ali was awarded the US Fulbright Fellowship to the University of Okayama, Japan, where he worked on mycoviruses (viruses infecting fungi). He has published 90 peer-reviewed articles, ten book chapters, and co-edited Pepper Virome: Diversity, Evolution and Management. Ali has been an invited speaker at 18 different institutions and 22 global conferences.

Klappentext

Learn to get the most out of your molecular biology experiments

This hands-on guide shows how to identify and characterize organisms on the molecular scale, understand how they work, locate a particular gene or disease's causal agents at the molecular level, and apply all of this knowledge to the real word issues. The only book of its kind, Experimental Techniques in Molecular Biology gathers all the information you need in one concrete source that covers the many techniques performed in a lab setting and are important for recombinant DNA technology. You will discover how to navigate different situations to target nucleic acids (single and double stranded RNA and DNA) or proteins from different living organisms.

The book shows, step by step, how to prepare solutions and buffers, and how to extract RNA, dsRNA, DNA, and protein from virus-infected plant tissues and fungi as well as from blood, animals, insects, bacteria, fungi, and nematodes. Isolation, characterization, quantification, and sequencing are covered in full detail. You will get background information on each technique, clear explanations of each method and why it is chosen, and the different solutions and their roles in the procedures.

• Features lab techniques not present in other core textbooks
• Includes 100 full-color illustrations
• Written by an experienced molecular biology academic