Recombinant DNA Principles and Methodologies

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.
Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!

Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as
plasmid biology
nucleic acid biochemistry
restriction enzymes
cloning strategies
gel electrophoresis
southern and northern blotting
preparation of probes
phage lambda biology
cosmids and genome analysis
cloned gene expression
polymerase chain reaction
conventional and automated DNA sequencing
site-directed mutagenesis
and more!
Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Auteur

James Greene (Catholic University, Washington D.C., USA)

Contenu

Why Cloning?, James J. Greene
Biochemistry of Nucleic Acids, Paul S. Miller
Enzymology of Recombinant DNA, Frank J. Castora
Basic Escherichia coli Genetics Useful for Recombinant DNA Techniques, Darren D. Sledjeski and Susan Garges
Formulation of Cloning Strategies, John B. Dame
Isolation and Purification of Nucleic Acids, James J. Greene
Gel Electrophoresis, Southern and Northern Analysis, James J. Greene and Frank J. Castora
Hybridization Probes, Frank J. Castora and James J. Greene
cDNA Cloning and the Construction of Recombinant DNA, Dante S. Zarlenga
Molecular Cloning by Plasmid Vectors, Paulina Balbás and Francisco Bolívar
Phage Lambda: Basic Biology and Use as a Vector, Susan Frackman and Michael Feiss
Cosmid Cloning, John Collins and Werner Lindenmaier
Multipurpose Cloning Vectors, August J. Sick, Joseph M. Fernandez, and Jay M. Short
Mammalian Expression Vectors, Ricky R. Hirschhorn and Nava Sarver
Approaches to the Expression of Foreign Genes, Allan R. Shatzman
Screening for Specific Recombinant Clones, Lucia Irene González-Villaseñor and Mark M. Manak
Principles and Applications of the Polymerase Chain Reaction, Garbriela S. Dveksler and Carl W. Dieffenbach
DNA Sequencing, Maurice A. Kashdan, Phillip T. Moen, Jr.,Venigalla B. Rao, and Bachtoi S. Rao
Site-Directed Mutagenesis, Alfred Johnson and Marvin Reitz