Enzymatic Fuel Cells

Summarizes research encompassing all of the aspects required to
understand, fabricate and integrate enzymatic fuel cells

• Contributions span the fields of bio-electrochemistry and
  biological fuel cell research

• Teaches the reader to optimize fuel cell performance to achieve
  long-term operation and realize commercial applicability

• Introduces the reader to the scientific aspects of
  bioelectrochemistry including electrical wiring of enzymes and
  charge transfer in enzyme fuel cell electrodes

• Covers unique engineering problems of enzyme fuel cells such as
  design and optimization

Auteur

HEATHER R. LUCKARIFT is the Senior Research Scientist for
Universal Technology Corporation at the Air Force Civil Engineer
Center (formerly the Microbiology & Applied Biochemistry team
at the Air Force Research Laboratory). She is the author of over
fifty peer-reviewed publications and invited reviews.

PLAMEN ATANASSOV is a Professor of Chemical & Nuclear
Engineering and the founding director of The University of New
Mexico Center for Emerging Energy Technologies. He was the
principal investigator on an Air Force Office of Scientific
Research Multi-University Research Initiative program:
"Fundamentals and Bioengineering of Enzymatic Fuel
Cells." He is the author of more than 220 publications,
including twelve reviews.

GLENN R. JOHNSON is the Chief Scientist and founder of
Hexpoint Technologies and the former principal investigator of the
Microbiology & Applied Biochemistry team within the Air Force
Research Laboratory. He is the author of over fifty peer-reviewed
publications and invited reviews.

Résumé

Summarizes research encompassing all of the aspects required to understand, fabricate and integrate enzymatic fuel cells

• Contributions span the fields of bio-electrochemistry and biological fuel cell research
• Teaches the reader to optimize fuel cell performance to achieve long-term operation and realize commercial applicability
• Introduces the reader to the scientific aspects of bioelectrochemistry including electrical wiring of enzymes and charge transfer in enzyme fuel cell electrodes
• Covers unique engineering problems of enzyme fuel cells such as design and optimization

Contenu

Preface xv

Contributors xvii

1 Introduction 1
Heather R. Luckarift, Plamen Atanassov, and Glenn R. Johnson

List of Abbreviations, 3

2 Electrochemical Evaluation of Enzymatic Fuel Cells and Figures of Merit 4
Shelley D. Minteer, Heather R. Luckarift, and Plamen Atanassov

2.1 Introduction, 4

2.2 Electrochemical Characterization, 5

2.2.1 Open-Circuit Measurements, 5

2.2.2 Cyclic Voltammetry, 5

2.2.3 Electron Transfer, 6

2.2.4 Polarization Curves, 6

2.2.5 Power Curves, 8

2.2.6 Electrochemical Impedance Spectroscopy, 8

2.2.7 Multienzyme Cascades, 8

2.2.8 Rotating Disk Electrode Voltammetry, 9

2.3 Outlook, 9

Acknowledgment, 10

List of Abbreviations, 10

References, 10

3 Direct Bioelectrocatalysis: Oxygen Reduction for Biological Fuel Cells 12
Dmitri M. Ivnitski, Plamen Atanassov, and Heather R. Luckarift

3.1 Introduction, 12

3.2 Mechanistic Studies of Intramolecular Electron Transfer, 13

3.2.1 Determining the Redox Potential of MCO, 13

3.2.2 Effect ofpHand Inhibitors on the Electrochemistry ofMCO, 17

3.3 Achieving DET of MCO by Rational Design, 18

3.3.1 Surface Analysis of Enzyme-Modified Electrodes, 20

3.3.2 Design of MCO-Modified Biocathodes Based on Direct Bioelectrocatalysis, 21

3.3.3 Design of MCO-Modified Air-Breathing Biocathodes, 22

3.4 Outlook, 25

Acknowledgments, 26

List of Abbreviations, 26

References, 27

4 Anodic Catalysts for Oxidation of Carbon-Containing Fuels 33
Rosalba A. Rincón, Carolin Lau, Plamen Atanassov, and Heather R. Luckarift

4.1 Introduction, 33

4.2 Oxidases, 34

4.2.1 Electron Transfer Mechanisms of Glucose Oxidase, 34

4.3 Dehydrogenases, 35

4.3.1 The NADH Reoxidation Issue, 35

4.3.2 Mediators for Electrochemical Oxidation of NADH, 37

4.3.3 Electropolymerization of Azines, 38

4.3.4 Alcohol Dehydrogenase as a Model System, 41

4.4 PQQ-Dependent Enzymes, 42

4.5 Outlook, 44

Acknowledgment, 45

List of Abbreviations, 45

References, 45

5 Anodic Bioelectrocatalysis: From Metabolic Pathways to Metabolons 53
Shuai Xu, Lindsey N. Pelster, Michelle Rasmussen, and Shelley D. Minteer

5.1 Introduction, 53

5.2 Biological Fuels, 53

5.3 Promiscuous Enzymes Versus Multienzyme Cascades Versus Metabolons, 55

5.3.1 Promiscuous Enzymes, 55

5.3.2 Multienzyme Cascades, 56

5.3.3 Metabolons, 56

5.4 Direct and Mediated Electron Transfer, 57

5.5 Fuels, 58

5.5.1 Hydrogen, 58

5.5.2 Ethanol, 58

5.5.3 Methanol, 60

5.5.4 Methane, 61

5.5.5 Glucose, 61

5.5.6 Sucrose, 65

5.5.7 Trehalose, 65

5.5.8 Fructose, 67

5.5.9 Lactose, 68

5.5.10 Lactate, 68

5.5.11 Pyruvate, 69

5.5.12 Glycerol, 70

5.5.13 Fatty Acids, 70

5.6 Outlook, 72

Acknowledgment, 72

List of Abbreviations, 73

References, 73

6 Bioelectrocatalysis of Hydrogen Oxidation/Reduction by Hydrogenases 80
Anne K. Jones, Arnab Dutta, Patrick Kwan, Chelsea L. McIntosh, Souvik Roy, and Sijie Yang

6.1 Introduction, 80

6.2 Hydrogenases, 81

6.3 Biological Fuel Cells Using Hydrogenases: Electrocatalysis, 85

6.4 Electrocatalysis by Functional Mimics of Hydrogenases, 92

6.4.1 [FeFe]-Hydrogenase Models, 92

6.4.2 [NiFe]-Hydrogenase Models, 95

6.4.3 Incorporation of Outer Coordination Sphere Features, 97

6.5 Outlook, 97 Acknowl...