Cell Separation

Cell Separation: Methods and Selected Application, Volume 3 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including lymphoma cells, lectins, purification of cells from lung tumors, macrophage electrophoretic migration test, tissue heterogeneity, and characteristics of cultured cells.

Organized into 13 chapters, this volume begins with an overview of the approaches in examining particular cell-surface properties and their role in the metastatic process. This text then examines lectins as important tools for identification and separation of cells, particularly of lymphocyte subpopulations. Other chapters consider the various methods that have been used to disperse rat pituitary tissue into single cells. This book discusses as well the different methods for isolating type II cells. The final chapter deals with the significance of having cell cultures homogeneous for a given cell type.

This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Contenu

Contributors
Preface
Contents of Previous Volumes

1. Separation of Malignant Lymphoid Cells by Countercurrent Distribution
   I. Introduction
   II. Experimental Methods
   III. Fractionation of RAW117 Lymphoma Cells
   IV. Discussion of the Technique
   References
2. Use of Lectins for Separation of Cells
   I. Introduction
   II. Methodology
   III. Selected Applications
   IV. Concluding Remarks
   References
3. Enzymatic Disaggregation of Human Bronchogenic Carcinomas followed by Velocity Sedimentation of Cells
   I. Introduction
   II. Enzymatic Disaggregation of Tumors
   III. Suspensions of Tumors: Storage and Composition
   IV. Purification of Cells from Lung Tumors
   V. Concluding Remarks
   References
4. Macrophage Electrophoretic Migration (MEM) Test with Some Illustrative and Heuristic Applications
   I. Historical Introduction
   II. Basic Requirements
   III. Preparation of Macrophages
   IV. Preparation of Lymphocytes
   V. Procedure: Cancer
   VI. Applications
   References
5. Implementation of Microtechniques to Resolve Tissue Heterogeneity
   I. Introduction
   II. Description of Microtechniques
   III. Cell Differentiation in the Cellular Slime Mold Dictyostelium discoideum
   IV. Adaptation of the Micromethods to Human Mammary Tumors
   V. Conclusions
   References
6. In Vitro Isolation of Malignant Cells from Small Cell Carcinomas
   I. Introduction
   II. Establishment of Cell Lines
   III. Characteristics of Cultured Cells
   IV. Summary
   References
7. Computer-Controlled Cell Electrophoresis Microscope
   I. Introduction
   II. Design of the Automated Electrophoresis Microscope System (AEMS)
   III. Features Important to the Operation of the AEMS
   IV. Conclusions
   References
8. Separation of Cells from the Rat Anterior Pituitary Gland
   I. Cellular Organization of the Rat Anterior Pituitary Gland
   II. Preparation of Rat Pituitary Cell Suspensions
   III. Methods Used to Differentiate Pituitary Cell Types
   IV. Review of Pituitary Cell Separation Studies
   V. Separated Pituitary Cells: Applications
   VI. Concluding Remarks
   References
9. Isolation of Type II Pneumocytes and Their Application in the Study of Pulmonary Metabolism
   I. Introduction
   II. Overview-Type II Cell Isolation
   III. Method of Isolation
   IV. Applications
   V. Conclusion
   References
10. Selective Media
    I. Introduction
    II. Biological Basis for Selective Growth
    III. Families of Cells with Similar Growth Requirements
    IV. Selective Growth of Human Epidermal Keratinocytes
    V. Techniques for Achieving Selective Growth
    VI. Future Prospects
    References
11. Cell Labeling and Separation Using Immunospecific Microspheres
    I. Introduction
    II. General Considerations
    III. Cell-Specific Ligands
    IV. Microspheres for Cell Labeling and Separation
    V. Cell-Labeling Methods
    VI. Applications of Microspheres in Cell Separations
    VII. Summary and Concluding Remarks
    References
12. Collagenase and the Isolation of Cells from Bone
    I. Cells of the Osteoblast Line
    II. Cells of the Osteoclast Line
    III. Isolation of Calvarial Cells
    IV. Clostridium histolyticum Collagenase
    V. Cell Separation Techniques
    VI. Culture of Human Bone Cells
    VII. Freezing Cells for Storage
    References
13. Cell Separation by Biological Methods
    I. Introduction
    II. Differential Culture Procedures
    III. Selective Reagents
    IV. Selective Media
    V. Discussion
    References
    Index