Receptors, Membrane Transport and Signal Transduction

A NATO Advanced study Institute on "Receptors, Membrane Trans port and Signal Transduction", was held on the Island of Spet sai, Greece, from August 16-27, 1988, in order to consider re cent developments in membrane receptor research, membrane trans port and signal transduction mechanisms. These topics were put in the larger context of current knowledge on the structure and function of membranes; connections between different fields of research were established by in-depth dis cussions of energy transduction and transport mechanisms. The general principles of regulation by signal transduction and protein phosphorylation/dephosphorylation were presented in the context of specific cellular processes. Discussions included al so the role of protein tyrosine kinases which are structurally related to oncogene products and, therefore, implicated in va rious aspects of cell development and transformation. This book presents the content of the major lectures and a se lection of the most relevant posters presented during the course of the Institute. The book is intended to make the proceedings of the Institute accessible to a larger audience and to offer a comprehensive account of those topics on receptors, membrane transport and signal transduction that were discussed extensive ly during the course of the Institute. February 1989 The Editors CONTENTS I. G-PROTEINS, ADENYLATE CYCLASE AND PROTEIN PHOSPHORYLATION Selective regulation of G proteins by Cell surface receptors ......... .

Texte du rabat

This book summarizes developments in membrane receptor research, membrane transport and signal transduction mechanisms. The general principles of regulation by signal transduction and protein phosphorylation/dephosphorylation are presented in the context of specific cellular processes. This also includes the role of protein tyrosine kinases which are structurally related to oncogene products and, therefore, implicated in various aspects of cell development and transformation. All topics are placed in the larger context of the current knowledge on the structure and function of membranes; connections between different fields of research are established by in-depth discussions on energy transduction and transport mechanisms.

Contenu
I. G-Proteins, Adenylate Cyclase and Protein Phosphorylation.- Selective regulation of G proteins by Cell surface receptors.- Regulation of adenylate cyclase in mammalian cells and Saccharomyces cerevisiae.- Protein kinases, Protein phosphatases and the regulation of glycogen metabolism.- Phosphorylase kinase and protein kinase C:Functional similaritie.- The use of specific antisera to locate functional domains of guanine nucleotide binding proteins.- Calcium inhibits GTP-binding proteins in squid photoreceptors.- Degradation of the invasive adenylate cyclase toxin of bordetella pertussis by the eukaryotic target cell-lysate.- Identification and characterization of adenylate cyclases in various tissues by monoclonal antibodies.- The role of G-proteins in exocytosis.- Hydrophobic interactions in the calcium-and phospholipid dependent activation of protein kinase C.- Activation of transducing by aluminum or beryllium fluoride complexes.- II. Membrane Receptors and Neurotransmitters.- Glutamate receptors and glutamatergic synapses.- Mechanisms of glutamate exocytosis from isolated nerve terminals.- Characteristics of the epidermal growth factor receptor.- Three-dimensional structural models for EGF and insulin receptor interactions and signal transduction.- Potentiation of neurotransmitter release coincides with potentiation of phosphatidyl inositol turnover A possible in vitro model for long term potentiation (LTP).- Purification and localization of kainate binding protein in pigeon cerebellum.- The Norepinephrine analog meta-iodo-benzylguanidine (MIBG) as a substrate for mono (ADP-ribosylation).- The synaptic vesicle vesamicol (AH5183) receptor contains a low affinity acetylcholine binding site.- Purification of the D-2 dopamine receptor and characterization of itssignal transduction mechanism.- Downregulation of M1 and M2 muscarinic receptor subtypes in Y1 mouse adenocarcinoma cells.- Uptake of GABA and L-glutamate into synaptic vesicles.- Deactivation of laminin-specific cell-surface receptors accompanies immobilization of myoblasts during differentiation.- III. Membrane Transport and Bioenergetics.- Signal Transduction in Halobacteria.- Control of bacterial growth by membrane processes.- Carbonylcyanide-3-chlorophenylhydrazone, a prototype agent for the selective killing of cells in acidic regions of solid tumours.- Ca2+ and pH interactions in thrombin stimulated human platelets..- Structure-function relationships of the pCloDF13 encoded BRP.- Binding of a Bacillus Thuiingiensis delta endotoxin to the midgut of the tobacco hornwoim (Manduca sexta).- Functional reconstitution of photosynthetic reaction centre complexes from Rhodopseudomonas Palustris.- Na+/H+ exchange in cardiac cells: Implications for electrical and mechanical events during intracellular pH changes.- Receptor-mediated inhibition of reproductive activity in a schistosome-infected freshwater snail.- NMR study of gramicidin cation trasnport across and integration into a lipid membrane.