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Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Contenu
Part I. Screening and Design Purification Strategies
Nikolaos Labrou
Ignacio Amadeo, Laura Mauro, Eduardo Ortí, and Guillermina Forno
A. S. Rathore and R. Bhambure
A. S. Rathore and S. Muthukumar
Charlotte Cabanne and Xavier Santarelli
Sunil Kumar and Narayan S. Punekar
Sunil Chhatre and Daniel G. Bracewell
Part II. Low-Resolution Protein Purification Methods
Zhengrong GU
Oscar Aguilar and Marco Rito-Palomares
A. S. Rathore and R. Bhambure
Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta
Ario de Marco
Victor Morais, Patricia Berasain, and Hugo Massaldi
Part III: High-Resolution Protein Purification Methods
Ana Sofia Pina, Íris L. Batalha, and Ana Cecília A. Roque
Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa
Sharon Williams, Phil Morton, and Dev Baines
My Hedhammar, Johan Nilvebrant, and Sophia Hober
Johan Nilvebrant, Mikael Åstrand, and Sophia Hober
Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servé W.M. Kengen, and John van der Oost
Isabel T. Sousa and M. Ângela Taipa
Evangelia Chronopoulou and Nikolaos E. Labrou
S. A. Camperi, M. C. Martínez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone
E.G. Vlakh, G.A. Platonova, and T.B. Tennikova
Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlüter
Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization
Catalin E. Doneanu, Weibin Chen, and St John Skilton
Jens Sproß and Andrea Sinz
Cosima Damiana Calvano, Cristina De Ceglie, and Carlo G. Zambonin
Sona Krizkova, Marketa Ryvolova, Michal Masarik, Ondrej Zitka, Vojtech Adam, Jaromir Hubalek, Tomas Eckschlager, and Rene Kizek
Protein Structure Validation and Analysis with X-Ray Crystallography
Anastassios C. Papageorgiou and Jesse Mattsson
Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes
Annette C. Moser, Benjamin White, and Frank A. Kovacs
Ken-ichi Izutsu
Pavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, and Eduarda Dráberová
Part V. Applications/Case Studies
Karolina Corin, Lotta T. Tegler, and Sotirios Koutsopoulos
Pierpaolo Falcicchio, Mark Levisson, Servé W.M. Kengen, Sotirios Koutsopoulos , and John van der Oost
Sushrut Arora, B. Vijayalakshmi Ayyar, and Richard O'Kennedy
Evangelos Topakas and Paul Christakopoulos
Anna Moosmann, Egbert Müller, and Heiner Böttinger
W. Yao, J. Zhu, and B. Sun