Plant Hormone Receptors

The Nato Advanced Research Workshop on Plant Hormone Receptors was held at the Physik Zentrum in Bad Honnef near Bonn, August 18-22, 1986. This workshop was mainly supported by the Nato Scientific Affairs Division and additionally cosponsered by Hoechst AG, Frankfurt and BASF AG, Ludwigshafen. The workshop aimed at focusing research on plant hormone recep tors. It should provide an opportunity to all who work in this field to report on their very recent data and to discuss their results with the most competent' colleagues. The total number of participants was limited to 30 to ensure personal contact and intensive discussions. Everyone had to either give a lecture or practical course. One half of the participants were invited, the other was selected by applications. Plant hormone receptors are assumed to exist but clear results are still rare. Nevertheless encouraging results have been published over the last years. Receptors for animal hormones and neuronal transmitters are well characterized, both structu rally and functionally. Therefore scientists dealing with recep tors for steroid hormones - Prof. E.E. Baulieu, Paris and Prof. J. R. Gustafsson, Huddinge - and for acetylcholine - Prof. A. Maelicke, Dortmund - were invited to participate in the workshop.

Texte du rabat

The NATO Advanced Research Workshop on Plant Hormone Receptors, held in Bad Honnef in August 1986, provided an opportunity for scientists working in this field to present their most recent data and to discuss their results with the most competent of their colleagues. The report contains short reviews and new results of the knowledge on plant hormone recognition by plant cells and on the molecular mechanism of their action. The contributions include: - cytosolic and membrane associated auxin receptors and their possible functions, - auxin transport carriers, - cytokinin binding proteins, - ethylene binding proteins, - gibberellin binding proteins, - fusicoccin binding proteins. The report includes a methodical appendix for the most useful methods, employed in research work within this field.

Contenu
I. Hormone Recognition.- 1. Too many Binding Proteins, not enough Receptors?.- 2. Hormone Recognition in Plants.- II. Auxin Receptors.- 3. Can Auxin Receptors be Purified by Affinity Chromatography?.- 4. The Auxin Receptor in Corn Coleoptiles.- 5. Cytosolic and Membrane-Bound High-Affinity Auxin-Binding Proteins in Tobacco.- 6. Soluble Auxin-Binding: Is there a Correlation between Growth-Stage dependent High-Affinity Auxin-Binding and Auxin Competence?.- 7. Phytohormone-Receptors from Tobacco Crown Gall Tissues.- III. Auxin Transport Carrier.- 8. Auxin Transport: Binding of Auxins and Phytotropins to the Carriers. Accumulation into and Efflux from Membrane Vesicles.- 9. Preliminary Separation of Pea Stem NPA Receptors by High Performance Ion Exchange Chromatography.- 10. Effect of Surface and Membrane Potentials on IAA Uptake and Binding by Zucchini Membrane Vesicles.- 11. Modifications of Auxin Efflux Carrier in the Auxin Transport System by Diethyl Ether and Ethylene.- IV. Fusicoccin Receptor.- 12. Search for Endogenous Ligands to Fusicoccin Binding Sites.- 13. The Interaction of Fusicoccin with Specific Binding Sites.- V. Auxin Receptor and Phospholipids.- 14. About the Search for the molecular Action of High-Affinity Auxin-Binding Sites on Membrane-Localized Rapid Phosphoinositide Metabolism in Plant Cells.- 15. Hormone Receptor Manipulation by Hydrostatic Pressure: Interaction between Ca2+, Membrane Components and Phosphatidylinositol in Pea Foliage Microsomes.- 16. Stimulation of In Vitro H+ Transport in Zucchini Microsomes by the Ether Lipid Platelet Activating Factor and a Soluble Protein.- VI. Cytokinin Receptor.- 17. Immunocytological Localization of a wheat embryo Cytokinin Binding Protein and its Homology with Proteins in other Cereals.- 18. Studies on Cytokinin Binding Proteins.- VII. Gibberellin Receptor.- 19. The Gibberellin Receptor.- VIII. Ethylene Receptor.- 20. Ethylene Binding Sites.- 21. Ethylene Binding and Evidence that Binding In Vivo and In Vitro is to the Physiological Receptor.- 22. Ethylene-Induced Growth in Amphibious Plants.- Techniques in Plant Hormone Receptor Research.- 1. Affinity Chromatography is a Powerful Tool to Prepare Auxin Receptors.- Klämbt, D., Löbler, M..- 2. Soluble Auxin Binding Proteins in Pea: Preparation and Binidng Assays.- Hajek, K., Jacobsen, H.-J., Hess, D..- 3. Extraction of Auxin-Binding Proteins from Tobacco.- Teigen van, H. J., Mennes, A. M., Nakamura, C., Linde van der, P. C. G., Zaal van der, E. J., Quint, A., Libbenga, K. R..- 4. Methods for Membrane Preparation, Auxin and Phytotropin Binding, In Vitro Auxin Transport.- Hertel, R..- 5. Study of Solubilized Pea NPA Receptors using High Performance Ion Exchange Chromatography (HPIEC).- Short, T. W., Jacobs, M..- 6. Extraction and Assay of Gibberellin-Binding Proteins from Cucumber and Pea.- Yalpani, N., Liu, Z. H., Srivastava, L. M..- 7. Ethylene Binding and Metabolism in Pisum Sativum L. cv Alaska.- Sanders, I. O., Robertson, D. R., Smith, A. R., Hall, M. A..- 8. Purification of the Ethylene-Binding Component from Mung Bean Sprouts and Seeds.- Sisler, E. C..- 9. Characterizations and Purification of an Ethylene Binding Component from Developing Cotyledons of Phaseolus Vulgaris L..- Williams, R. A. N., Smith, A. R., Hall, M. A..