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This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.
Includes cutting-edge methods and protocols for studying peptide antibodies
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Contenu
Peptide Antibodies: Past, Present, and Future
Gunnar Houen
The Structure of Natural and Recombinant Antibodies
Hui Ma and Richard O'Kennedy
Prediction of Antigenic B and T Cell Epitopes Via Energy Decomposition Analysis: Description of the Web-Based Prediction Tool BEPPE
Claudio Peri, Oscar C. Solé, Dario Corrada, Alessandro Gori, Xavier Daura, and Giorgio Colombo
Prediction of Antibody Epitopes
Morten Nielsen and Paolo Marcatili
Fmoc Solid-Phase Peptide Synthesis
Paul Robert Hansen and Alberto Oddo
Peptide-Carrier Conjugation
Paul Robert Hansen
Solid Phase Peptide-Carrier Conjugation
Gunnar Houen and Dorthe T. Olsen
Analysis of Peptides and Conjugates by Amino Acid Analysis
Peter Højrup
Characterization of Synthetic Peptides by Mass Spectrometry
Bala K. Prabhala, Osman Mirza, Peter Højrup, and Paul Robert Hansen
Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis
Karin Hjernø and Peter Højrup
Polyclonal Peptide Antisera
Tina H. Pihl, Kristin E. Engelhart, and Gunnar Houen
Production and Screening of Monoclonal Peptide Antibodies
Nicole Hartwig Trier, Anne Mortensen, Annette Schiolborg, and Tina Friis
Production of Epitope-Specific Antibodies by Immunization with Synthetic Epitope Peptide Formulated with CpG-DNA-Liposome Complex Without Carriers
Dongbum Kim, Younghee Lee, and Hyung-Joo Kwon
Thioredoxin-Displayed Multipeptide Immunogens
Angelo Bolchi, Elena Canali, Andrea Santoni, Gloria Spagnoli, Daniele Viarisio, Rosita Accardi, Massimo Tommasino, Martin Müller, and Simone Ottonello
The Purification of Natural and Recombinant Peptide Antibodies by Affinity Chromatographic Strategies
Hui Ma and Richard O'Kennedy
Isolation of Camelid Single-Domain Antibodies Against Native Proteins Using Recombinant Multivalent Peptide Ligands
Norah A. Alturki, Kevin A. Henry, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi
Generation of TCR-Like Antibodies Using Phage Display
Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung
Structural Characterization of Peptide Antibodies
Anna Chailyan and Paolo Marcatili
Automated High Throughput Mapping of Linear B Cell Epitopes Using a Statistical Analysis of High Density Peptide Microarray Data
Thomas Østerbye and Søren Buus
Characterization of Peptide Antibodies by Epitope Mapping Using Resin-Bound and Soluble Peptides
Nicole Hartwig Trier
Screening and Characterization of Linear B-Cell Epitopes by Biotinylated Peptide Libraries
Ida Rosenkrands and Anja Olsen
Bead-Based Peptide Arrays for Profiling the Specificity of Modification State-Specific Antibodies
Angela Filomena, Yvonne Heubach, Markus F. Templin, Thomas O. Joos, Nicole Schneiderhan-Marra, and Oliver Poetz
Surface Plasmon Resonance Method to Evaluate Anti-Citrullinated Protein/Peptide Antibody Affinity to Citrullinated Peptides
Feliciana Real-Fernández, Giada Rossi, Filomena Panza, Federico Pratesi, Paola Migliorini, and Paolo Rovero
Specificity Analysis of Histone Modification Specific Antibodies or Reading Domains on Histone Peptide Arrays
Goran Kungulovski, Ina Kycia, Rebekka Mauser, and Albert Jeltsch
Prion-Specific Antibodies Produced in Wild-Type Mice
Peter M.H. Heegaard, Ann-Louise Bergström, Heidi Gertz Andersen, and Henriette Cordes
Immunoblotting with Peptide Antibodies: Differential Immunoreactivities Caused by Certain Amino Acid Substitutions in a Short Peptide, and Possible Effects of Differential Refolding of the Peptide on a Nitrocellulose or PVDF Membrane
Takenori Yamamoto, Taisuke Matsuo, Atsushi Yamamoto, Ryohei Yamagoshi, Kazuto Ohkura, Masatoshi Kataoka, and Yasuo Shinohara
Immunocytochemical and Immunohistochemical Staining with Peptide Antibodies
Tina Friis, Klaus Boberg Pedersen, David Hougaard, and Gunnar Houen
Designing B-Cell Epitopes for Immunotherapy and Subunit Vaccines
Harinder Singh, Sudheer Gupta, Ankur Gautam, and Gajendra P.S. Raghava
Enterovirus-Specific Anti-Peptide Antibodies
Chit Laa Poh, Katherine Kirk, Hui Na Chua, and Lara Grollo
Therapeutic HIV Peptide Vaccine
Anders Fomsgaard