Nitrogen Metabolism in Plants

This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Contenu
An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants.- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues.- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions.- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples.- Measurement of S-Nitrosoglutathione Reductase Activity in Plants.- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia.- Enzymatic Conversions of Glutamate and -Aminobutyric Acid as the Indicators of Plant Stress Response.- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae.- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes.- Methods for Estimation of Nitrogen Components in Plantsand Microorganisms.- A Precise Method for Analysing Nitrogen Use in Foxtail Millet.- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria.- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR.- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies.