Informationen zum Autor Editor-in-Chief, Dr. Michael C. Flickinger is the Associate Director for Academic Programs of the Golden LEAF Biomanufacturing Training and Education Center (BTEC) and a Professor of Microbiology and Chemical and Biomolecular Engineering at North Carolina State University, Raleigh. Dr. Flickinger was Director of the University of Minnesota's Institute for Advanced Studies in Biological Process Technology, which was later renamed the Biotechnology Institute ), and a Professor of Biochemistry, Molecular Biology and Biophysics at the University of Minnesota, St. Paul. Prior to coming to Minnesota Dr. Flickinger was Director of the Fermentation Program at the National Cancer Institute in Frederick, Maryland. Dr. Flickinger received his academic training at the University of Wisconsin, Madison.?(B.S.?(1973) in microbial biochemistry;? M.S. (1975) and Ph.D.?(1977) in pharmaceutical biochemistry.)? He did his postdoctoral work at the Laboratory for Renewable Resources Engineering? in the School of Chemical Engineering, Purdue University. He is Editor-in-Chief of the Encyclopedia of Industrial Biotechnology (Wiley, 2010) and Co-Editor of the Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation (Wiley, 1999). Klappentext Biotechnology represents a major area of research focus, and many universities are developing academic programs in the field. This guide to biomanufacturing contains carefully selected articles from Wiley's Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology as well as new articles (80 in all,) and features the same breadth and quality of coverage and clarity of presentation found in the original. For instructors, advanced students, and those involved in regulatory compliance, this two-volume desk reference offers an accessible and comprehensive resource. Zusammenfassung Biotechnology represents a major area of research focus, and many universities are developing academic programs in the field....

Auteur

Editor-in-Chief, Dr. Michael C. Flickinger is the Associate Director for Academic Programs of the Golden LEAF Biomanufacturing Training and Education Center (BTEC) and a Professor of Microbiology and Chemical and Biomolecular Engineering at North Carolina State University, Raleigh.
Dr. Flickinger was Director of the University of Minnesota's Institute for Advanced Studies in Biological Process Technology, which was later renamed the Biotechnology Institute ), and a Professor of Biochemistry, Molecular Biology and Biophysics at the University of Minnesota, St. Paul. Prior to coming to Minnesota Dr. Flickinger was Director of the Fermentation Program at the National Cancer Institute in Frederick, Maryland.
Dr. Flickinger received his academic training at the University of Wisconsin, Madison.?(B.S.?(1973) in microbial biochemistry;? M.S. (1975) and Ph.D.?(1977) in pharmaceutical biochemistry.)? He did his postdoctoral work at the Laboratory for Renewable Resources Engineering? in the School of Chemical Engineering, Purdue University.
He is Editor-in-Chief of the Encyclopedia of Industrial Biotechnology (Wiley, 2010) and Co-Editor of the Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation (Wiley, 1999).

Contenu
VOLUME I: EXPRESSION SYSTEMS & PROCESS DEVELOPMENT

PREFACE xi

CONTRIBUTORS xiii

PART I INTRODUCTION 3

PART II INDUSTRIAL CELL GROWTH AND GENE EXPRESSION SYSTEMS 7

1 Animal Cells, Suspension Culture 9
John R. Birch

2 Baculovirus Expression Systems 19
Robert D. Possee, Richard B. Hitchman, and Linda A. King

3 Baculovirus Kinetics, Insect Culture 33
Leslie Chan, Steve Reid, and Lars Keld Nielsen

4 Cell Culture, Aseptic Techniques 49
John M. Davis and Kevin L. Shade

5 Cell Cycle in Bioprocesses 71
Mariam Naciri and Mohamed Al-rubeai

6 Cell Growth and Protein Expression Kinetics 85
Dhinakar S. Kompala

7 Cell Viability Measurement 97
Ning Wei and Benjamin Sommer

8 Contamination Detection in Animal Cell Culture 105
Carol Mclean and Colin Harbour

9 Culture Collections and Biological Resource Centers (BRCs) 131
David Smith

10 Culture Preservation 153
Robert L. Gherna

11 Expression and Secretion of Heterologous Proteins, Bacillus and Other Gram-Positive Bacteria 163
Boyke Bunk, Rebekka Biedendieck, Dieter Jahn, and Patricia S. Vary

12 Gene Expression in Human Cells 181
Marco A. Cacciuttolo, Gene Lee, John Chon, and John Lewis

13 Gene expression in Pichia and other methylotroph yeast 195
Koti Sreekrishna

14 Gene Expression in Recombinant Animal Cells and Transgenic Animals 213
Richard M. Twyman and Bruce Whitelaw

15 Inoculum Expansion Methods, Animal Cell Lines 297
Claudia Kloth, Glenn Maclsaac, Haile Ghebremariam, and Alahari Arunakumari

16 Insect Cell Culture 311
Someet Narang, Erik M. Whiteley, Sunyia Hussain, and Michael J. Betenbaugh

17 Kinetics of Microbial Growth 331
Nicolai S. Panikov

18 Microalgae, Mass Culture Methods 371
Emílio Molina Grima, Jose María Fernández Sevilla, and Francisco Gabriel Acién Fernández

19 Microbial Growth Measurement 399
Arthur L. Koch

20 Microbial Media Composition 413
Rosalie J. Cote

21 Microscopic Characterization of Cells 437
Erwin Huebner

22 Mycoplasma Contamination of Cell Cultures 467
Cord C. Uphoff and Hans G. Drexler

23 Protein Glycosylation: Analysis, Characterization, and Engineering 489
Mikael R. Andersen, Jong Hyun Nam, and Susan T. Sharfstein

24 Secretion of Heterologous Proteins, Gram Positive Bacteria 543
Eric Morello, Isabelle Poquet, Philippe Langella

25 Soluble Protein Expression in Bacteria 557
Catherine H. Schein

PART III MEDIA, CELL LINES AND PROCESS DEVELOPMENT 579

26 Animal Cell Culture Media 581
Natarajan Vijayasankaran, Jincai Li, Robert Shawley, Aaron Chen, Masaru Shiratori, Martin Gawlitzek, Feng Li, Robert Kiss, and Ashraf Amanullah

27 Animal Cell Culture, Effects of Osmolality and Temperature 599
James C. Warren and Shyamsundar Subramanian

28 Animal Cell Stability 617
Martin S. Sinacore, Timothy S. Charlebois, Denis Drapeau, Mark Leonard, Scott Harrison, and S. Robert Adamson

29 Animal Cell Types, Hybridomas 635
K. Heilmann and B. Micheel

30 Antibody Production, Human Recombinant 645
Stefan Dübel

31 Antifoams and Pluronic Polyols, Cell Protection 663
David W. Murhammer

32 Biominiaturization of Bioreactors 669
Michael A. Hanson and Govind Rao

33 Inoculum Preparation 699
Craig J.L. Gershater

34 Microcarrier Culture 711
Susan T. Sharfstein and Christian Kaisermayer

35 Monoclonal Antibody Production, Cell Lines 733
Julia F. Markusen and David K. Robinson

36 Plant Cell Culture, Laboratory Techniques 747
Mark Richard Fowler

37 Scale-Up of Biotechnological Processes 759
Marko Zlokarnik

VOLUME II: EQUIPMENT, PROCESS DESIGN, SENSING, CONTROL, AND cGMP OPERATIONS