Methods in Membrane Lipids

This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico . Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Contenu

Introduction to Membrane Lipids.- Introduction: Membrane properties (Good) for Life.- Introduction To Fluorescence Probing Of Biological Membranes.- The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs).- Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells.- Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning.- Asymmetric Giant Lipid Vesicle Fabrication.- Cholesterol Depletion using Methyl-b-cyclodextrin.- A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems.- Imaging Membrane Order using Environmentally Sensitive Fluorophores.- 3D Super-resolution Imaging by Localisation Microscopy.- Electron Microscopy Methods for Studying Plasma Membranes.- Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy.- Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order.- Scanning Fluorescence Correlation Spectroscopy on Biomembranes.- X-ray Diffraction of Lipid Model Membranes.- Solid State NMR of Lipid Model Membranes.- Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications.- The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells.- Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations.- Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers.