Methods in Chemosensory Research

"The authors are to be congratulated for doing step-by-step 'how-to' descriptions of many current techniques in the field, something any new researcher or student will find very usefulfound the writing style to be consistently readable and informative. Much practical information is givenThis book represents the kind of basic and well-written laboratory manual and overview textbook longed for in every field. I recommend it to graduate students, laboratory supervisors, and researchers"Perceptual Motor Skills, 2002

Auteur

Miguel A. L. Nicolelis, Sidney A. Simon

Texte du rabat

Methods in Chemosensory Research describes new molecular, electrophysiological, engineering, genetic, behavioral, psychophysical, and imaging techniques that have recently been adapted to investigate the basic neuronal mechanisms underlying chemoreception. Written by leaders in the field of neuronal chemoreception specializing in olfaction, gustation, baroreception, and chemical irritation caused by environmental pollutants, this unique book combines novel methods to investigate all aspects of taste and olfaction. These methods range from the molecular level to the investigation of individual cell taste and olfactory epithelia.

Résumé
Describes molecular, electrophysiological, engineering, genetic, behavioral, psychophysical, and imaging techniques that have been adapted to investigate the basic neuronal mechanisms underlying chemoreception. This book investigates various aspects of taste and olfaction.

Contenu

BEHAVIORAL STUDIES. Psychophysical Measurement of Oral Chemesthesis. Behavioral Methods in Olfactory Research with Rodents. MOLECULAR AND CELL BIOLOGY OF TASTE AND OLFACTION. Recordings from Vertebrate Olfactory Receptor Neurons: From Isolated Cells to Intact Epithelial Preparations. Recordings from Olfactory Receptor Neurons in the Rat in Vivo. Voltage-Sensitive and Calcium-Sensitive Dye Imaging of Activity in the Olfactory Bulb; Presynaptic Inhibition, Maps of Receptor Cell Input and Oscillations. Gustatory System Development: New Experimental Approaches in Amphibian and Mammalian Embryos. Mammalian Taste Receptors. Researching Isolated Taste Receptor Cells: Deciphering Transduction Cascades with Patch-Clamp And Calcium: Imaging Techniques. Rapid Kinetic Measurements in Chemosensory Systems. Electrophysiological Recordings of Mammalian Taste. HIGER-ORDER STUDIES IN TASTE AND OLFACTION. Activation of Neurons in the Trigeminal Subnucleus Caudalis (Vc) By Irritant Chemical Stimulation: Extracellular Single-Unit Recording and c-fos Immunohistochemical Methods. Methodological Considerations for Electrophysiological Recording and Analysis of Taste-Responsive Neurons in the Brainstem of the Rate. Electrophysiological Analysis of Olfactory Coding in the CNS. Electrophysiological Studies of Gustation in Awake Rats. Recording from Single Neurons in the Primary Taste Cortex of the Alert Macaque. Olfactory Learning and the Neurophysiological Study of Rat Prefrontal Function. Olfactory Event-Related Potentials. Application of Functional MRI in Olfactory Studies. Functional Imaging of Olfactory Activation in the Human Brain.