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Screening, Production and Characterization of Lipase by Microorganisms

  • Couverture cartonnée
  • 144 Nombre de pages
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Lipases are hydrolytic enzymes that hydrolyse triglycerides to free fatty acids and glycerol. Isolation and screening of lipolytic... Lire la suite
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Description

Lipases are hydrolytic enzymes that hydrolyse triglycerides to free fatty acids and glycerol. Isolation and screening of lipolytic microorganisms from different environmental samples of different ecological habitat have been left relatively unexplored. Thus, a total of 37 bacteria 17 fungi were isolated from different environmental samples and screenedfor lipase production. Three bacterial isolates and fungal isolates each with maximum lipase activity on lipase assay medium were selected for further study. Fermentation pattern was studied by varying process parameters like initial pH of production media, incubation temperature, different substrates, substrate concentration and agitation in order to maximize extracellular lipase production. Coconut oil was found to induce best lipase production in bacteria and fungi in which Acinetobacter sp. (1.45 U/ml) and Hypocrea patella (10.00 U/ml) had the highest lipase production. Fermentation period between 3-7days was found to be the best for lipase production in fungi and 12-24 h in bacteria.The study has proven that optimization of growth parameters in a suitable fermentation medium has significant effect on improved lipase production.

Auteur

Haliru Musa is an Assistant Lecturer in the Department of Biological Science, Kwara Sate Polytechnic, Ilorin, Nigeria since 2010 and presently a PhD research student in School of Bioprocess Engineering, Universiti Malaysia Perlis, Malaysia. His research interest is on,"Improved Enzymatic Esters production".

Informations sur le produit

Titre: Screening, Production and Characterization of Lipase by Microorganisms
Auteur:
Code EAN: 9783659714610
ISBN: 978-3-659-71461-0
Format: Couverture cartonnée
Editeur: LAP Lambert Academic Publishing
Genre: Biologie
nombre de pages: 144
Poids: g
Taille: H220mm x B220mm x T150mm
Année: 2017