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Dictyostelium discoideum Protocols

  • Livre Relié
  • 492 Nombre de pages
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The book offers an introduction to the amoebozoa, presents large-scale analysis methods made possible by the completion of the Dic... Lire la suite
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Description

The book offers an introduction to the amoebozoa, presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence, and details molecular genetics techniques, cell biological, biochemical and biophysical methods.

Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of D. discoideum in recent years.

The second edition of Dictyostelium discoideum Protocols incorporates the most recent developments in a number of fields. The book is divided into four parts. The first part provides an introduction to the amoebozoa and community resources. The second part presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence. The third part is dedicated to molecular genetics techniques, cell biological, biochemical and biophysical methods. The chapters in the fourth part describe the use of Dictyostelium as a model system to study vesicle formation, trafficking and infection by bacterial pathogens.

Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Dictyostelium discoideum Protocols, Second Edition seeks to aid scientists in the application of innovating techniques to study a range of fundamental biological processes in this attractive model organism.



Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Contenu
1. The Amoebozoa Christina Schilde and Pauline Schaap 2. The Model Organism Dictyostelium discoideumSalvatore Bozzaro 3. Comparative Genomics of the DictyostelidsWilliam F. Loomis 4. One Stop Shop for Everything Dictyostelium: DictyBase and the Dicty Stock Center in 2012Petra Fey, Robert J. Dodson, Siddhartha Basu and Rex L. Chisholm 5. Fluorescent Reporters and Methods to Analyze Fluorescent SignalsAnnette Müller-Taubenberger and Hellen C. Ishikawa-Ankerhold 6. Collection and Cultivation of Dictyostelids from the WildTracy E. Douglas, Debra A. Brock, Boahemaa Adu-Oppong, David C. Queller and Joan E. Strassmann 7. Identification and Verification of microRNAs by High-throughput SequencingJimmie Hällman, Lotta Avesson, Johan Reimegård, Max Käller and Fredrik Söderbom 8. Transcriptional Profiling of Dictyostelium with RNA SequencingEdward Roshan Miranda, Gregor Rot, Marko Toplak, Balaji Santhanam, Tomaz Curk, Gad Shaulskyand Blaz Zupan 9. Analysis of Chromatin Organization by Deep Sequencing TechnologiesJames L. Platt, Nick A. Kent, Adrian J. Harwood, and Alan R. Kimmel 10. Pharmacogenetics of Resistance to Cisplatin and Other Anti-cancer Drugs and the Role of Sphingolipid MetabolismStephen Alexander, William S. Swatson, Hannah Alexander 11. N-glycomic and Glycoproteomic Studies in the Social Amoebae Christa L. Feasley, Alba Hykollari, Katharina Paschinger, Iain B. H. Wilson, and Christopher M. West 12. Measuring Cheating, Fitness, and Segregation in D. discoideumNeil J Buttery, Jeff smith, David C Queller and Joan E Strassmann 13. The Application of the Cre-loxP System for Generating Multiple Knock-Out and Knock-In Targeted LociJan Faix, Joern Linkner, Benjamin Nordholz, James L. Platt, Xin-Hua Liao, and Alan R. Kimmel 14. Extrachromosomal Inducible ExpressionDouwe M. Veltman1 and Peter J.M. Van Haastert215. Isolation of Dictyostelium Nuclei for Light and Electron MicroscopyPetros Batsios, Otto Baumann, Ralph Gräf and Irene Meyer 16. Investigation of DNA Repair Pathway ActivityAnne-Marie C. Couto, Nicholas D. Lakin and Catherine J. Pears 17. Transcript Localization in D. discoideum Cells by RNA FISHPatrick Hofmann, Janis Kruse and Christian Hammann 18. Analysis of Mitochondrial Gene ExpressionJessica E. Accari, Sam Manna, Paul R. Fisher, and Christian Barth 19. Mitochondrial Respiratory Complex Function and the Phenotypic Consequences of DysfunctionSarah J. Annesley, Sergio Carilla-Latorre, Ricardo Escalante and Paul R. Fisher 20. Micropipette Aspiration for Studying Cellular Mechanosensory Responses and Mechanics Yee-Seir Kee and Douglas N. Robinson 21. Quantitative Analysis of Phagocytosis and Phagosome MaturationNatascha Sattler, Roger Monroy and Thierry Soldati 22. Setting Up and Monitoring an Infection of Dictyostelium discoideum with MycobacteriaSonia Arafah, Sébastien Kicka, Valentin Trofimov, Monica Hagedorn, Nuria Andreu, Siouxsie Wiles, Brian Robertson, and Thierry Soldati 23. Isolation of Pathogen-containing Vacuoles Olga Shevchuk and Michael Steinert 24. Immuno-magnetic Purification of Fluorescent Legionella-containing VacuolesIvo Finsel, Christine Hoffmann and Hubert Hilbi 25. Secretory Lysosomes in Dictyostelium: Visualization, Characterization and DynamicsWanessa C. Lima and Pierre Cosson 26. Monitoring Autophagy in DictyosteliumAna Mesquita, Javier Calvo-Garrido, Sergio Carilla-Latorre and Ricardo Escalante

Informations sur le produit

Titre: Dictyostelium discoideum Protocols
Éditeur:
Code EAN: 9781627033015
ISBN: 1627033017
Format: Livre Relié
Editeur: Humana Press
nombre de pages: 492
Poids: 1114g
Taille: H260mm x B183mm x T31mm
Année: 2013
Auflage: 2nd ed. 2013

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